지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.9
- 수록면
- 205 - 216 (12page)
- DOI
- 10.5145/ACM.2024.27.3.4
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초록· 키워드
오류제보하기
Background: Unlike the Mycoplasma IST2 kit (bioMérieux), the Mycoplasma IST3 kit hasbeen updated to comply with the standardized antimicrobial susceptibility test (AST) methodfor Ureaplasma spp. (Up) and Mycoplasma hominis (Mh). We aimed to verify the use of theMycoplasma IST3 kit for genital mycoplasma cultures.
Methods: From September 2023 to January 2024, the R1 medium remaining after inoculationwith IST2 was refrigerated until the next day. For IST2-positive samples, 300 μL of residual R1medium was inoculated into the IST3. Species identification, enumeration, and AST resultsobtained using IST3 were compared with those obtained using IST2.
Results: A total of 48 IST2-positive samples were inoculated into IST3, including 35, 1, and12 Up-only, Mh-only, and both Up- and Mh-positive samples, respectively. Among Up-onlysamples, 2.8%, 91.4%, and 100.0% were susceptible to ciprofloxacin, tetracycline, anderythromycin, respectively. With IST3, 45 (93.8%) samples grew genital mycoplasmas; 42(89.4%) of the 47 Up-positive samples and 6 (46.2%) of the 13 Mh-positive samples showedgrowth of the same organisms. All seven samples that failed to grow Mh were from mixedcultures, of which four Mh concentrations of < 104/mL. Up was susceptible to levofloxacin,tetracycline, and erythromycin at the rates of 64.3 %, 88.1 %, and 95.2 %, respectively.
Conclusion: IST3 showed good performance in detecting genital Mycoplasma except forits tendency to not detect Mh of low concentrations in mixed cultures. IST3 is preferable toIST2 because it can accurately screen for erythromycin resistance in Up and reduce falseresistances for fluoroquinolone.
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