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논문 기본 정보

자료유형
학술저널
저자정보
Minjoong Joo (Basilbiotech) Jeong-Hun Mok (Sungkyunkwan University) Van-An Duong (Gachon University) Jong-Moon Park (Basilbiotech) Hookeun Lee (Gachon University)
저널정보
한국질량분석학회 Mass Spectrometry Letters Mass Spectrometry Letters Vol.15 No.2
발행연도
2024.6
수록면
69 - 78 (10page)

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초록· 키워드

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The advancement of high-throughput omics technologies and systems biology is essential for understanding complex biological mechanisms and diseases. The integration of proteomics and metabolomics provides comprehensive insights into cellular functions and disease pathology, driven by developments in mass spectrometry (MS) technologies, including electrospray ionization (ESI). These advancements are crucial for interpreting biological systems effectively. However, integrating these technologies poses challenges. Compared to genomic, proteomics and metabolomics have limitations in throughput, and data integration. This review examines developments in MS equipped electrospray ionization (ESI), and their importance in the effective interpretation of biological mechanisms. The review also discusses developments in sample preparation, such as Simultaneous Metabolite, Protein, Lipid Extraction (SIMPLEX), analytical techniques, and data analysis, highlighting the application of these technologies in the study of cancer or Huntington's disease, underscoring the potential for personalized medicine and diagnostic accuracy. Efforts by the Clinical Proteomic Tumor Analysis Consortium (CPTAC) and integrative data analysis methods such as O2PLS and OnPLS extract statistical similarities between metabolomic and proteomic data. System modeling techniques that mathematically explain and predict system responses are also covered. This practical application also shows significant improvements in cancer research, diagnostic accuracy and therapeutic targeting for diseases like pancreatic ductal adenocarcinoma, non-small cell lung cancer, and Huntington's disease. These approaches enable researchers to develop standardized protocols, and interoperable software and databases, expanding multi-omics research application in clinical practice.

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Abstract
Introduction
1. MS based proteo-metabolomics
2. Sample preparation
3. Data acquisition and feature identification
4. Integrative analysis of proteomics and metabolomics
Conclusion
References

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