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이 논문의 연구 히스토리 (4)
2013
Diagnostic Utility of Multiplex Reverse Transcriptase-Polymerase Chain Reaction in Hematologic Malignancies
김민진
의학과
2013.01
학위논문
The Utility of the Multiplex Reverse Transcriptase- Polymerase Chain Reaction Assay in the Detection of Hematologic Malignancies
김민진
,
조선영
,
박태성
외 2명
Annals of Laboratory Medicine
2013.01
학술저널
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초록· 키워드
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Background: In order to assess the diagnostic utility of multiplex reverse transcriptase-polymerase chain reaction (multiplex RT-PCR) for hematologic malignancies, this study compares its results with those of conventional cytogenetics. Additionally, results derived from the previously used single reverse transcriptase-nested polymerase chain reaction (single RT-Nested PCR) were investigated to see if there were any differences.
Materials and Methods: Among patients diagnosed with hematologic malignancies between January 2005 to June 2012 at Kyung Hee Medical Center, retrospective study was conducted using data of conventional cytogenetics and PCR. Specimens collected between January 2005 and May 2008 underwent single RT-Nested PCR for 3 frequently reported gene rearrangements (AML1/ETO, PML/RARA, BCR/ABL1) while those collected between June 2008 and June 2012 were tested by HemaVision (DNA Technology, Aarhus, Denmark).
Results: Gene rearrangements were found in 40.0% (26/65) of the sample under single RT-Nested PCR and in 51.9% (42/81) under multiplex RT-PCR. There were seven patients whose test results in single RT-Nested PCR were different from chromosomal study where three of them turned out to be normal and the other four showed translocations other than the three designated gene rearrangements. For those subject to multiplex RT-PCR, three patients turned out to be normal in chromosomal study while they were found to have gene rearrangements.
Conclusion: Findings suggest that multiplex RT-PCR can detect various hematologic malignancy related gene translocations that are difficult to find with chromosomal study and therefore should be considered as a useful method for future diagnoses of hematologic malignancies.
Materials and Methods: Among patients diagnosed with hematologic malignancies between January 2005 to June 2012 at Kyung Hee Medical Center, retrospective study was conducted using data of conventional cytogenetics and PCR. Specimens collected between January 2005 and May 2008 underwent single RT-Nested PCR for 3 frequently reported gene rearrangements (AML1/ETO, PML/RARA, BCR/ABL1) while those collected between June 2008 and June 2012 were tested by HemaVision (DNA Technology, Aarhus, Denmark).
Results: Gene rearrangements were found in 40.0% (26/65) of the sample under single RT-Nested PCR and in 51.9% (42/81) under multiplex RT-PCR. There were seven patients whose test results in single RT-Nested PCR were different from chromosomal study where three of them turned out to be normal and the other four showed translocations other than the three designated gene rearrangements. For those subject to multiplex RT-PCR, three patients turned out to be normal in chromosomal study while they were found to have gene rearrangements.
Conclusion: Findings suggest that multiplex RT-PCR can detect various hematologic malignancy related gene translocations that are difficult to find with chromosomal study and therefore should be considered as a useful method for future diagnoses of hematologic malignancies.
목차
1. Abstract 12. Introduction 33. Materials and Methods 53.1 Patients and specimens 53.2 Cytogenetic analysis 53.3 Fluorescence in situ hybridization 63.4 RNA extraction 63.5 Single RT-Nested PCR 63.6 Multiplex RT-PCR 84. Results 104.1 Patient characteristics 104.2 Detection of translocations by single RT-Nested PCR and multiplex RT-PCR 124.3 Discrepancy between RT-PCR and karyotyping 145. Discussion 166. References 207. Korean abstract 23
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