지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 임성우
- 발행연도
- 2014
- 저작권
- 동국대학교 논문은 저작권에 의해 보호받습니다.
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Objectives : This study was carried out to investigate the protective effect of Coptidis Rhizoma steamed with rice wine(CR) against gastroduodenal mucosal injury through inhibition of iNOS activation.
Methods : In in vitro experiment, LPS-induced RAW 264.7 macrophages were treated with CR(0.4, 0.6, 0.8, 1.0 ㎎/㎖) and iNOS mRNA expression and nitric oxide(NO) production were measured. In in vivo experiment, normal group mice were not treated with neither etanol nor CR. mice were orally administrated with etanol in both etanol treated group(control group), CR treated after etanol treatment group(sample group). While control group mice were treated with nothing else, sample group mice were orally administrated with CR five hours after etanol administration. After administration, gastroduodenal mucosa was observed histopathologically and iNOS, NF-κB activation were observed immunohistochemically.
Results : In in vitro experiment, iNOS mRNA expression and NO production in LPS-induced RAW 264.7 macrophages were decreased by CR dose-dependently. In in vivo experiment, gastroduodenal mucosal injury was repaired by CR and iNOS, NF-κB activation in gastroduodenal mucosa were decreased by CR.
Conclusions : Coptidis Rhizoma steamed with rice wine has protective effect against gastroduodenal mucosal injury through inhibition of iNOS activation.
Methods : In in vitro experiment, LPS-induced RAW 264.7 macrophages were treated with CR(0.4, 0.6, 0.8, 1.0 ㎎/㎖) and iNOS mRNA expression and nitric oxide(NO) production were measured. In in vivo experiment, normal group mice were not treated with neither etanol nor CR. mice were orally administrated with etanol in both etanol treated group(control group), CR treated after etanol treatment group(sample group). While control group mice were treated with nothing else, sample group mice were orally administrated with CR five hours after etanol administration. After administration, gastroduodenal mucosa was observed histopathologically and iNOS, NF-κB activation were observed immunohistochemically.
Results : In in vitro experiment, iNOS mRNA expression and NO production in LPS-induced RAW 264.7 macrophages were decreased by CR dose-dependently. In in vivo experiment, gastroduodenal mucosal injury was repaired by CR and iNOS, NF-κB activation in gastroduodenal mucosa were decreased by CR.
Conclusions : Coptidis Rhizoma steamed with rice wine has protective effect against gastroduodenal mucosal injury through inhibition of iNOS activation.
목차
Ⅰ. 서 론 = 1Ⅱ. 실 험 = 21. 실험 재료 = 21) 세포주 = 22) 동물 = 23) 약재 = 22. 실험 방법 = 41) in vitro = 4(1) iNOS mRNA 측정을 위한 RT-PCR = 4(2) Nitrite Assay = 52) in vivo = 6(1) 위?십이지장 점막 손상 유발 = 6(2) 酒蒸黃連 추출액의 투여 = 6(3) 조직표본 = 6(4) 위 점막 관찰 = 6① 위 점막 점액 분비 세포 = 6② 위 점막 내 NF-κB, iNOS 활성 = 6(5) 십이지장 점막 관찰 = 8① 파넷 세포 = 8② 십이지장 점막 내 NF-κB, iNOS 활성 = 83) 영상분석과 통계처리 = 8Ⅲ. 결 과 = 81. iNOS mRNA 발현과 NO 생성 억제 효과 = 81) iNOS mRNA 발현 억제 = 82) NO 생성 억제 = 82. 위?십이지장 점막 손상 보호 효과 = 101) 위 점막 = 10(1) 위 점막 손상 회복 = 10(2) 점액 분비 세포 손상 회복 = 10(3) 위 점막 내 NF-κB, iNOS 활성 억제 = 12① NF-κB 활성 억제 = 12② iNOS 활성 억제 = 122) 십이지장 점막 = 15(1) 십이지장 점막 손상 회복 = 15(2) 파넷 세포 손상 회복 = 15(3) 십이지장 점막 내 NF-κB, iNOS 활성 억제 = 17① NF-κB 활성 억제 = 17② iNOS 활성 억제 = 17Ⅳ. 고 찰 = 20Ⅴ. 결 론 = 24참 고 문 헌 = 25ABSTRACT = 28
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