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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 임성우
- 발행연도
- 2016
- 저작권
- 동국대학교 논문은 저작권에 의해 보호받습니다.
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Objectives
The aim of this study is to investigate the effects of Gaejigayonggolmoryo-Tang (GYT) on the ulcerative colitis induced by dextran sulfate sodium (DSS) in mice.
Methods
Colitis induced by free drinking of 5% DSS was tested on six-week-old male ICR mice. The mice were divided into two groups: prevention group and treatment group. Prevention group was once again divided into three groups -Sample group, Control group and Normal group. The ''Sample group'' was treated with GYT for 14 days before it was given 5% DSS for 5 days. The ''Control group'' was given water instead of the medication for 14 days before the 5 days of 5% DSS. The ''Normal group'' was not given 5% DSS for comparison purpose.
The treatment group was also divided into three groups: Sample group, Control group and Normal group. The ''Sample group'' was treated with GYT for 3 days after it was given 5% DSS for 5 days. The ''Control group'' was given water instead of the medication for 3 days after the 5 days of 5% DSS. The ''Normal group'' was not given 5% DSS for comparison purpose.
Result
Inhibiting effects on inflammatory enzymes investigated by cellular experiment show GYT inhibits the expression of COX-2 and iNOS, and the production of NO. Based on the primary cellular experiment, the effects on ulcerative colitis induced by DSS of mice tissues were investigated. The inhibiting effects on the damages of tissues, the expression of inflammatory enzymes such as NF-κB p65, COX-2 and iNOS, and apoptosis were confirmed. In cellular experiment, GYT was more effective in inhibiting the expression of COX-2 than the expression of iNOS. However, GYT was evidently effective on tissues to inhibit the expression of COX-2.
Conclusions
It is supposed that Gaejigayonggolmoryo-Tang has both preventive and therapeutic effects on ulceratice colitis induced by DSS. Also, it is worthy of research and development as a COX-2 inhibitor and a potential drug for inflammatory bowel diseases from natural products. Further investigations for exact mechanisms will be needed.
Key words:
Gaejigayonggolmoryo-Tang, ulcerative colitis, NF-κB p65, COX-2, iNOS and apoptosis
The aim of this study is to investigate the effects of Gaejigayonggolmoryo-Tang (GYT) on the ulcerative colitis induced by dextran sulfate sodium (DSS) in mice.
Methods
Colitis induced by free drinking of 5% DSS was tested on six-week-old male ICR mice. The mice were divided into two groups: prevention group and treatment group. Prevention group was once again divided into three groups -Sample group, Control group and Normal group. The ''Sample group'' was treated with GYT for 14 days before it was given 5% DSS for 5 days. The ''Control group'' was given water instead of the medication for 14 days before the 5 days of 5% DSS. The ''Normal group'' was not given 5% DSS for comparison purpose.
The treatment group was also divided into three groups: Sample group, Control group and Normal group. The ''Sample group'' was treated with GYT for 3 days after it was given 5% DSS for 5 days. The ''Control group'' was given water instead of the medication for 3 days after the 5 days of 5% DSS. The ''Normal group'' was not given 5% DSS for comparison purpose.
Result
Inhibiting effects on inflammatory enzymes investigated by cellular experiment show GYT inhibits the expression of COX-2 and iNOS, and the production of NO. Based on the primary cellular experiment, the effects on ulcerative colitis induced by DSS of mice tissues were investigated. The inhibiting effects on the damages of tissues, the expression of inflammatory enzymes such as NF-κB p65, COX-2 and iNOS, and apoptosis were confirmed. In cellular experiment, GYT was more effective in inhibiting the expression of COX-2 than the expression of iNOS. However, GYT was evidently effective on tissues to inhibit the expression of COX-2.
Conclusions
It is supposed that Gaejigayonggolmoryo-Tang has both preventive and therapeutic effects on ulceratice colitis induced by DSS. Also, it is worthy of research and development as a COX-2 inhibitor and a potential drug for inflammatory bowel diseases from natural products. Further investigations for exact mechanisms will be needed.
Key words:
Gaejigayonggolmoryo-Tang, ulcerative colitis, NF-κB p65, COX-2, iNOS and apoptosis
목차
Ⅰ. 서론 01Ⅱ. 재료 및 방법 031. 재료 031) 배양세포 032) 동물 033) 약재 034) 탕약 추출물 제조 052. 방법 061) Dextran Sulfate Sodium(DSS)에 의한 대장염 유발 062) Cell viability assay 073) NO 생성 측정 074) 염증효소 유전자 발현 측정 075) 조직표본 준비 096) 조직화학 및 면역학적 염색 097) 세포자연사 118) 영상분석 및 통계처리 11Ⅲ. 결과 121. RAW264.7세포를 이용한 염증억제 효과1) 桂枝加龍骨牡蠣湯이 RAW 264.7 세포생존율에 미치는 영향 122) RAW264.7 세포에서의 NO 생성 억제 효과 143) RAW264.7 세포에서의 염증효소 유전자 발현 억제 효과 162. 동물실험을 이용한 桂枝加龍骨牡蠣湯의 효과 181) 예방 효과 18(1) 형태적 변화 18(2) 상피세포 보호 효과 20(3) 염증성 발현인자 억제 효과 22① NF-κB p65 억제 효과 22② COX-2 억제 효과 24③ iNOS 억제 효과 26(4) 세포자연사 억제 효과 282) 치료 효과 30(1) 형태적 변화 30(2) 상피세포 보호 효과 32(3) 염증성 발현인자 억제 효과 34① NF-κB p65 억제 효과 34② COX-2 억제 효과 36③ iNOS 억제 효과 38(4) 세포자연사 억제 효과 40Ⅳ.고찰 42Ⅴ.결론 49참 고 문 헌 50A BSTRACT 55
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