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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 이종훈
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- 2020
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초록· 키워드
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CHAPTER 1. The Anti-photoaging Effects of Pueraria montana var. lobata Extract on UVB-irradiated Human Dermal Fibroblasts 1
Ⅰ. Introduction 1
Ⅱ. Materials & Methods 8
1) Chemicals and Reagents 8
2) Preparation of Pueraria montana var. lobata Extract 8
3) Cell Culture and UVB-irradiation 8
4) Cell Viability Assay 9
5) Measurement of Intracellular ROS Production 9
6) Measurement of Total Collagens 10
7) Hyaluronic Acid Assay 10
8) Western Blot Analysis 11
9) Immunocytochemistry 11
10) Statistical Analysis 12
Ⅲ. Results & Discussion 13
1) Cytotoxicity of PLE on HDFs 13
2) Effects of PLE on Collagen Production 15
3) Effects of PLE on Cellular Hyaluronic Acid Synthesis 17
4) Inhibition of Intracellular ROS Production of PLE 19
5) Effects of PLE on Expression of Antioxidant Enzymes 21
6) Antioxidant Effects of PLE and Correlation with
Collagen Synthesis 23
7) Discussion 25
Ⅳ. Conclusion 26
CHAPTER 2. The Antioxidant Effects of Lava Seawater in vitro 28
Ⅰ. Introduction 28
Ⅱ. Materials & Methods 32
1) Chemicals and Reagents 32
2) Preparation of Lava Seawater and Cell Line 32
3) Cell Culture and UVB-irradiation 32
4) Cell Viability Assay 33
5) Total Collagen Measurement 34
6) Oil Red O Staining 34
7) Cytosolic and Nuclear Protein Extraction 35
8) Western Blot Analysis 35
9) Statistical Analysis 35
Ⅲ. Results & Discussion 37
1) Cytotoxicity of LSW on HDFs and hADSCs 37
2) Effects of LSW on Collagen Production 39
3) Expression of Antioxidant Enzymes on HDFs 41
4) Antioxidant Enzymes Expression Mechanism 44
5) Inhibition Effects on Adipogenic Differentiation
on hADSCs 46
6) Antioxidant Enzymes Expression on hADSCs 49
Ⅳ. Conclusion 52
Reference 53
Ⅰ. Introduction 1
Ⅱ. Materials & Methods 8
1) Chemicals and Reagents 8
2) Preparation of Pueraria montana var. lobata Extract 8
3) Cell Culture and UVB-irradiation 8
4) Cell Viability Assay 9
5) Measurement of Intracellular ROS Production 9
6) Measurement of Total Collagens 10
7) Hyaluronic Acid Assay 10
8) Western Blot Analysis 11
9) Immunocytochemistry 11
10) Statistical Analysis 12
Ⅲ. Results & Discussion 13
1) Cytotoxicity of PLE on HDFs 13
2) Effects of PLE on Collagen Production 15
3) Effects of PLE on Cellular Hyaluronic Acid Synthesis 17
4) Inhibition of Intracellular ROS Production of PLE 19
5) Effects of PLE on Expression of Antioxidant Enzymes 21
6) Antioxidant Effects of PLE and Correlation with
Collagen Synthesis 23
7) Discussion 25
Ⅳ. Conclusion 26
CHAPTER 2. The Antioxidant Effects of Lava Seawater in vitro 28
Ⅰ. Introduction 28
Ⅱ. Materials & Methods 32
1) Chemicals and Reagents 32
2) Preparation of Lava Seawater and Cell Line 32
3) Cell Culture and UVB-irradiation 32
4) Cell Viability Assay 33
5) Total Collagen Measurement 34
6) Oil Red O Staining 34
7) Cytosolic and Nuclear Protein Extraction 35
8) Western Blot Analysis 35
9) Statistical Analysis 35
Ⅲ. Results & Discussion 37
1) Cytotoxicity of LSW on HDFs and hADSCs 37
2) Effects of LSW on Collagen Production 39
3) Expression of Antioxidant Enzymes on HDFs 41
4) Antioxidant Enzymes Expression Mechanism 44
5) Inhibition Effects on Adipogenic Differentiation
on hADSCs 46
6) Antioxidant Enzymes Expression on hADSCs 49
Ⅳ. Conclusion 52
Reference 53
목차
CHAPTER 1. The Anti-photoaging Effects of Pueraria montana var. lobata Extract on UVB-irradiated Human Dermal Fibroblasts 1Ⅰ. Introduction 1Ⅱ. Materials & Methods 81) Chemicals and Reagents 82) Preparation of Pueraria montana var. lobata Extract 83) Cell Culture and UVB-irradiation 84) Cell Viability Assay 95) Measurement of Intracellular ROS Production 96) Measurement of Total Collagens 107) Hyaluronic Acid Assay 108) Western Blot Analysis 119) Immunocytochemistry 1110) Statistical Analysis 12Ⅲ. Results & Discussion 131) Cytotoxicity of PLE on HDFs 132) Effects of PLE on Collagen Production 153) Effects of PLE on Cellular Hyaluronic Acid Synthesis 174) Inhibition of Intracellular ROS Production of PLE 195) Effects of PLE on Expression of Antioxidant Enzymes 216) Antioxidant Effects of PLE and Correlation with Collagen Synthesis 237) Discussion 25Ⅳ. Conclusion 26CHAPTER 2. The Antioxidant Effects of Lava Seawater in vitro 28Ⅰ. Introduction 28Ⅱ. Materials & Methods 321) Chemicals and Reagents 322) Preparation of Lava Seawater and Cell Line 323) Cell Culture and UVB-irradiation 324) Cell Viability Assay 335) Total Collagen Measurement 346) Oil Red O Staining 347) Cytosolic and Nuclear Protein Extraction 358) Western Blot Analysis 359) Statistical Analysis 35Ⅲ. Results & Discussion 371) Cytotoxicity of LSW on HDFs and hADSCs 372) Effects of LSW on Collagen Production 393) Expression of Antioxidant Enzymes on HDFs 414) Antioxidant Enzymes Expression Mechanism 445) Inhibition Effects on Adipogenic Differentiation on hADSCs 466) Antioxidant Enzymes Expression on hADSCs 49Ⅳ. Conclusion 52Reference 53
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