지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The osteoblastic cell activity is important for born formation, thus, this study was performed to investigation of that the effect of edible sources, Rubus coreanus Miquel (RCM), on the proliferation and differentiation of MC3T3-E1 osteoblastic like cell. The effects of RCM extract on cell proliferation were measured by MTT assay. At 1, 10 μg/mL of RCM extract treated, that were elevated of cell proliferation to 103 and 142% via control, respectively. And the cell differentiation were measured as alkaline phosphatase (ALP) activity at 3, 9, 18, and 27 days. As the results, the 10 μg/mL was increased ALP activity more than 2.6 times compared with control, 1.4 times via positive control at 27th day (p<0.05). The optical concentration of RC extract was rechecked by ALP staining and Alizarin Red staining for investigation of the induction of ALP activity, nodule formation by mineralization. mRNA expression analysis showed that the RCM (10μg/mL) increased in SOX9 as well as ALP in MC3T3-E1 cells. These results suggest that RC extract was stimulates the MC3T3-E1 cell proliferation and differentiation.
목차
Abstract
재료 및 방법
결과 및 고찰
요약
감사의 글
참고문헌
참고문헌 (41)
참고문헌 신청
[학술지(정기간행물)] - / 1996 / Phyto-estrogens in relation to cancer and other human health risks 55 : 399 ~ 417
[학술지(정기간행물)] - / 1986 / Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations : 143 ~ 154
[학술지(정기간행물)] - / 1997 / The prevention of treatment of age-related osteoporosis in the elderly by systemic recombinant growth factor therapy rhIGF-I or rhRGF- 242 : 285 ~ 290
[학술지(정기간행물)] - / 1987 / Evaluation of a tetrazolium based semiautomated colorimetric assay Assessment of chemosensitivity testing : 936 ~ 942
[학술지(정기간행물)] - / 1987 / Transforming growth Factor B is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fatal rat bone : 2869 ~ 2874
[학술지(정기간행물)] - / 1986 / Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations : 143 ~ 154
[학술지(정기간행물)] - / 1997 / The prevention of treatment of age-related osteoporosis in the elderly by systemic recombinant growth factor therapy rhIGF-I or rhRGF- 242 : 285 ~ 290
[학술지(정기간행물)] - / 1987 / Evaluation of a tetrazolium based semiautomated colorimetric assay Assessment of chemosensitivity testing : 936 ~ 942
[학술지(정기간행물)] - / 1987 / Transforming growth Factor B is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fatal rat bone : 2869 ~ 2874
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UCI(KEPA) : I410-ECN-0101-2009-470-014448585