지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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A bacterium producing five fibrinolytic isozymes was isolated from black bean chung kuk. The bacterium was identified as Bacillus subtilis BB-1 by 16s rDNA sequence homology search. A gene out of five fibrinolytic genes in the Bacillus subtilis BB-1 was cloned by shot-gun method. A Cla I DNA fragment of B. subtilis BB-1 chromosome was cloned in to pBluescript II SK(-) and showed the fibrinolytic activity to bacterial cells. The Cla I DNA fragment was sequenced and the sequences did not show homology with gene for protease or fibrinolytic enzyme genes in other organisms. The Cla I DNA fragment was reduced to 2,142 bp by activity-guided PCR cloning method. The optimum pH and temperature of the enzyme were 5.0 and 35℃, respectively. Substrate specificity of the fibrinolytic enzyme was detected in skim milk, casein, gelatin and blood agar plates. The activity of the enzyme was not detected with these substrates. Taken together, this enzyme is a new fibrinolytic enzyme and may be used to prevent thrombosis and arteriosclerosis.
목차
Abstract
재료 및 방법
결과 및 고찰
요약
감사의 글
참고문헌
참고문헌 (30)
참고문헌 신청
Astrup, T and S. Hullertz. 1952. The fibrin plate method for estimating fibrinolytic activity. The National Danish Society against Rheumatic Disease. 346-351.
Astrup, T. and Stermdorff, I. 1956. The plasminogen activator in animal tissue. ACTA physiol. scand., 363 250.
Bok-Nan Kim, Jong-Dai Kim, Seung-shi Ham, Yong-Sorn Choi and Sang-Young Lee. 1995. Effects of spice added natto supplementation on the Lipid Metabolism in Rats. J. Korean Soc. Food Nutr., 24(1), 121-126.
Bullock, W. O., J. M. Fernandez and J. M. Short. 1987. XL1-blue: A high efficiency plasmid transforming recA Escherichia coli strain with beta galactosidase selation. Bio Techiques 5, 376.
Birhbion, H. C and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res. 7, 1513-1523.
Astrup, T. and Stermdorff, I. 1956. The plasminogen activator in animal tissue. ACTA physiol. scand., 363 250.
Bok-Nan Kim, Jong-Dai Kim, Seung-shi Ham, Yong-Sorn Choi and Sang-Young Lee. 1995. Effects of spice added natto supplementation on the Lipid Metabolism in Rats. J. Korean Soc. Food Nutr., 24(1), 121-126.
Bullock, W. O., J. M. Fernandez and J. M. Short. 1987. XL1-blue: A high efficiency plasmid transforming recA Escherichia coli strain with beta galactosidase selation. Bio Techiques 5, 376.
Birhbion, H. C and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res. 7, 1513-1523.
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UCI(KEPA) : I410-ECN-0101-2009-470-015375254