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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 370 - 374 (5page)
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초록· 키워드
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A gene encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1 was cloned from genomic DNAs. DNA sequencing showed that the gene, aprE86-1,could direct the synthesis of a mature protein 275 amino acids in length after processing. When aprE86-1 was introduced into B. subtilis, a mature 27-kDa protein was produced as expected. The fibrinolytic activity of the B.
subtilis transformant (TF) was higher than that of B.
amyloliquefaciens CH86-1, showing the possibility of increasing the fibrinolytic activity of Bacillus strains through genetic engineering.
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등록된 정보가 없습니다.
참고문헌 (10)
참고문헌 신청
Ae Ran Lee / 2010 / Characterization of a 27 kDa Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1 Isolated from Cheonggukjang / Journal of the Korean Society for Ap
Bradford, M. M / 1976 / Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
CHOI, NACK-SHICK / 2005 / Identification of Recombinant Subtilisins / Journal of Microbiology and Biotechnology 15 (1) : 35 ~ 39
CHOI, NACK-SHICK / 2006 / Identification of Three Extracellular Proteases from Bacillus subtilis KCTC 3014 / Journal of Microbiology and Biotechnology 16 (3) : 457 ~ 464
Gun-Hee Kwon / 2008 / Heterologous Gene Expression of aprE2 Encoding a 29 kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716 / Food Science a
Bradford, M. M / 1976 / Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
CHOI, NACK-SHICK / 2005 / Identification of Recombinant Subtilisins / Journal of Microbiology and Biotechnology 15 (1) : 35 ~ 39
CHOI, NACK-SHICK / 2006 / Identification of Three Extracellular Proteases from Bacillus subtilis KCTC 3014 / Journal of Microbiology and Biotechnology 16 (3) : 457 ~ 464
Gun-Hee Kwon / 2008 / Heterologous Gene Expression of aprE2 Encoding a 29 kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716 / Food Science a
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