배나무잎 이상반점증상의 이병여부를 조기에 판별할 수 있는 가장 간편한 검정방법을 개발코자 시험하였다. 접목시기는 3월 하순이 가장 좋았으며 접목시기가 늦어질수록 병징발현율이 감소하였다. 접목방법은 2중절접, 2중삭아절접 순으로 양호하였으나 숙련된 기술이 필요한 이중절접방법보다 간편한 2중삭아절접방법이 대량검정에 적합하였다. 이상반점증상의 전염에 필요한 최소 접촉시간은 1일 이상이었고 칼루스가 형성되어 접목부위가 활착된 21일 이후에서 발병이 가장 높았다.
A simple grafting method was developed for the early detection of latent infections with the pear abnormal leaf spot disease which is presumed to be caused by a virus. The best grafting time for inoculation was late March, and the later the grafting time, the less the symptom development. The double grafting method induced the severest symptoms, and the double chip grafting method induced the less severe symptoms. However, the latter was more appropriate for the mass screening of the disease since it is simple and easy to perform, while the former needs some degree of skills for grafting. Successful transmissions of the causal agent by the grafting required at least 1-day contact period, and the highest level of the symptom development was achieved at more than 21-day contact period until the grafted tissues were fully united each other to form a callus.