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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The extraction and separation of glabridin from licorice root by HPLC was performed in this work. First, by investigating the different extraction solvents, extraction methods and extraction times, a one-hour ultrasonic extraction procedure with ethyl acetate as the extraction solvent was optimized. Then the ethyl acetate extraction was applied to RP-HPLC for separation of glabridin. The column efficiencies and resolutions were experimentally investigated with different mobile phase compositions. Baseline separation of glabridin was obtained under the mobile phase composition of 50/50 vol.% (ACN/water). The retention time of glabridin was 20.3 min. The peak of glabridin was collected from the HPLC elution for several times and identified by LC/MS. Under the optimum extraction and HPLC separation methods, 1.26 g of glabridin per kg licorice root could be extracted.
목차
Abstract
1. Introduction
2. Experimental Section
3. Results and Discussions
4. Conclusion
Acknowledgements
References
참고문헌 (9)
참고문헌 신청
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T. Fukai, K. Satoh, T. Nomura, H. Sakagamic, Fitoterapia, 74(7-8), 624-629 (2003).
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H. Hikino, In: H. Wagner, H. Hikino, N. R. Farnsworth (Eds.), “Recent research on oriental medicinal plants”, Economic and medicinal plant research. London, Academic Press, 1985.
T. Fukai, K. Satoh, T. Nomura, H. Sakagamic, Fitoterapia, 74(7-8), 624-629 (2003).
H. Hirosaki, N. Hiraoka, Y. Ikeshiro and H. Yamamoto, Plant Science, 116(2), 233-238 (1996).
J. Vaya, P. A. Belinky and M. Aviram, Free Radical Biology & Medicine, 23(2), 302-313 (1997).
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UCI(KEPA) : I410-ECN-0101-2009-433-017617383