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논문 기본 정보

자료유형
학술저널
저자정보
In-Jung Kim (제주대학교) Tae-Sik Nam (전북대학교) Chan-Shick Kim (전북대학교) Kwan-Jeong Song (제주대학교) Dong-Hoon Lee (농촌진흥청)
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.51 No.6
발행연도
2010.12
수록면
551 - 555 (5page)

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A cDNA clone (Zds) encoding zeta-carotene desaturase (ZDS) was isolated from the Citrus fruit cDNA library. Sequence analyses indicated that the clone contains an open reading frame encoding 570 amino acids. A genomic Southern blot analysis and phylogenic dendrogram indicated that the Zds gene is present as more than two isoforms in the Citrus genome. Sequence analyses revealed that the cDNA showed significant similarity to other dicotyledonous woody plants. An RNA blot analysis showed that the transcripts were expressed in all investigated tissues of Citrus. During the ripening of fruits, the signal of the Zds transcripts was very weak until the break stage, but its expression markedly increased to the maximum level at the last stage. A similar pattern was detected in peels of citrus fruits. Furthermore, the level of Zds transcripts was changed in the process of leaf development. The results suggest that Citrus Zds is one of the important regulatory enzymes in carotenoid accumulation especially in the last stage of fruit ripening.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2012-525-003764491