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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.4
- 수록면
- 21 - 27 (7page)
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이 논문의 연구 히스토리 (5)
2013
2011
Development of Ultra-Rapid Real-Time PCR Method for Detection of Black Queen Cell Virus
Mi-Sun Yoo
,
Sang-Hoon Han
,
Byoung-Su Yoon
Journal of Apiculture
2011.10
학술저널
Development of Ultra-Rapid Real-Time PCR Method for the Detection of Nosema
Mi-Sun Yoo
,
Van Phu Nguyen
,
Ji-Na No
외 4명
Journal of Apiculture
2011.04
학술저널
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초록· 키워드
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Nosema ceranae is a microsporidian parasite of the honeybee, Apis mellifera, that is widespread and causes extensive loss. A micro PCR-based detection method, termed ultra-rapid real-time PCR, was developed to rapidly and quantitatively detect Nosema ceranae in honeybees. A specific detection primer set (Nosema-180-F/R) was used to amplify a unique 180bp DNA fragment with a micro-scale chip-based real-time PCR system, (GenSpector<SUP>®</SUP> TMC-1000), which has uncommonly fast heating and cooling rates (10℃/second) and a small reaction volume (6㎕). Real-time PCR assays using serially diluted DNA templates showed a high sensitivity of 10² copies. As a result, the minimum detection time was 12 min and 19 sec (30 cycles), including a melting temperature analysis. Therefore, this novel detection method could be used as a rapid real-time PCR-based diagnostic tool and is expected to be applied to rapidly detect various pathogens.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
LITERATURE CITED
참고문헌 (31)
참고문헌 신청
Anderson, D.L. / 1992 / Reduced pollen collection by honeybee (Hymenoptera, Apidae) colonies infection with Nosema apis and sacbrood virus / J. Econ. Entomol. 85:47~51
Bailey, L. / 1955 / The infection of the ventriculus of the adult honeybee by Nosema apis (Zander) / Parasitology 45:86~94
Böttcher, F.K. / 1974 / Die tätigkeit der Bayersichen Landesanstalt für Bienenzuccht in Erlangen im Jahre / Imkerfreund 30:75~85
Buys, B. / 1977 / A nosema disease affecting honeybee brood / Apimondia Symposium of Bee Biology and Pathology :73~76
Cho, Y.K. / 2006 / Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus / BIOSENSORS & BIOELECTRONICS 21:2161~2169
Bailey, L. / 1955 / The infection of the ventriculus of the adult honeybee by Nosema apis (Zander) / Parasitology 45:86~94
Böttcher, F.K. / 1974 / Die tätigkeit der Bayersichen Landesanstalt für Bienenzuccht in Erlangen im Jahre / Imkerfreund 30:75~85
Buys, B. / 1977 / A nosema disease affecting honeybee brood / Apimondia Symposium of Bee Biology and Pathology :73~76
Cho, Y.K. / 2006 / Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus / BIOSENSORS & BIOELECTRONICS 21:2161~2169
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UCI(KEPA) : I410-ECN-0101-2013-527-000244589