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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 203 - 208 (6page)
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이 논문의 연구 히스토리 (5)
2013
2011
Development of Ultra-Rapid Real-Time PCR Method for Detection of Black Queen Cell Virus
Mi-Sun Yoo
,
Sang-Hoon Han
,
Byoung-Su Yoon
Journal of Apiculture
2011.10
학술저널
Development of Ultra-Rapid Real-Time PCR Method for the Detection of Nosema
Mi-Sun Yoo
,
Van Phu Nguyen
,
Ji-Na No
외 4명
Journal of Apiculture
2011.04
학술저널
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초록· 키워드
오류제보하기
A novel micro PCR-based detection method, termed ultra-rapid real-time PCR, was developted for the detection of black queen cell virus (BQCV) which is one of fatal viral pathogens to queen larvae. GenSpector® TMC-1000 is characterized by uncommonly fast heating and cooling rates (10℃ per second) as well as a small reaction volume (6㎕). A specific detection primer set (BQCV-RT-F/R) was used for amplification of a unique 159-bp DNA fragment using the micro-scale chip-based real-time PCR system. High sensitivities capable of detecting 10 copies of DNA were observed in the ultrarapid real-time PCR assays using serially diluted DNA templates and artificially contaminated DNA templates. In addition, this method was also applicable to honeybee samples. In applying the ultrarapid real-time PCR detection method to a BQCV-infected honeybee, the shortest detection time was 8 min 34 sec, including melting temperature analysis. Therefore, this novel detection method is one of the most rapid real-time PCR-based diagnostic tools available, and it is expected to be applicable to the rapid detection of various pathogens.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
LITERATURE CITED
참고문헌 (23)
참고문헌 신청
Allen, M. / 1996 / The incidence and world distribution of honey bee viruses / Bee World 77:141~162
Anderson, D.L. / 1993 / Pathogens and queen bees / Australasian Beekeeper 94:292~296
Bailey, L. / 1976 / Viruses attacking the honey bee / Adv. Virus Res. 20:271~304
Bailey, L. / 1977 / Two more small RNA viruses from honeybee and further observation on sacbrood and acute bee paralysis viruses / J. Gen. Virol. 37:175~182
Cho, Y.K. / 2006 / Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus / BIOSENSORS & BIOELECTRONICS 21:2161~2169
Anderson, D.L. / 1993 / Pathogens and queen bees / Australasian Beekeeper 94:292~296
Bailey, L. / 1976 / Viruses attacking the honey bee / Adv. Virus Res. 20:271~304
Bailey, L. / 1977 / Two more small RNA viruses from honeybee and further observation on sacbrood and acute bee paralysis viruses / J. Gen. Virol. 37:175~182
Cho, Y.K. / 2006 / Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus / BIOSENSORS & BIOELECTRONICS 21:2161~2169
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UCI(KEPA) : I410-ECN-0101-2013-527-000925078