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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.8
- 수록면
- 888 - 891 (4page)
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초록· 키워드
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Twenty-three fungal strains were isolated from meju that had originated from the Sunchang province, the famous location for making fermented soybean foods in Korea. The restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rDNA (ITS-RFLP) was applied to differentiate the isolated fungal strains. First, the ITS region by polymerase chain reaction (PCR) with specific primers was amplified and then cleaved the products with different restriction enzymes. Cleavage of the amplified fragments with the restriction enzymes AluI, HaeIII, HhaI, and TaqI revealed extensive polymorphisms. The ITS-RFLP results highly correlated with ITS sequence analysis. All of the 23 fungal strains were classified into 5 groups by ITS-RFLP analysis. Aspergillus oryzae was the major fungal strain isolated from Sunchang meju (12 out of 23), while Aspergillus fumigatus was the next most frequently isolated strain (7 out of 23). In contrast, it was found that Fusarium asiaticum, Aspergillus sydowii, and Arthrinium sp. were the minor fungal strains in meju.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgment
References
참고문헌 (20)
참고문헌 신청
Park KY , 2003 , Antimutagenic effects of doenjang (Korean fermented soypaste) and its active compounds , Mutat. Res 523-524 : 43 ~ 53
Kim YM , 1977 , Fungal microflora on Korean home-made meju , Korean J. Mycol 5 : 7 ~ 12
Lee SS , 1997 , Studies on the yellow fungal isolates (Aspergillus species) inhabiting at the cereals in Korea , Korean J. Mycol 25 : 35 ~ 45
de Souza-Motta CM , 2003 , Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin , Braz. J. Microbiol 34 : 273 ~ 280
Gardes M , 1996 , ITS-RFLP matching for identification of fungi , Method Mol. Biol 50 : 177 ~ 186
Kim YM , 1977 , Fungal microflora on Korean home-made meju , Korean J. Mycol 5 : 7 ~ 12
Lee SS , 1997 , Studies on the yellow fungal isolates (Aspergillus species) inhabiting at the cereals in Korea , Korean J. Mycol 25 : 35 ~ 45
de Souza-Motta CM , 2003 , Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin , Braz. J. Microbiol 34 : 273 ~ 280
Gardes M , 1996 , ITS-RFLP matching for identification of fungi , Method Mol. Biol 50 : 177 ~ 186
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UCI(KEPA) : I410-ECN-0101-2013-573-001008696