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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.9
- 수록면
- 235 - 241 (7page)
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Purpose: The mass cultivation of functional hepatocytes is a key factor of a bioartificial liver. Combining spheroid and microcarrier cultures has been applied for enhancing the cell viability and metabolic activities. Hence, the optimal number of hepatocytes per microcarrier was investigated.
Methods: Firstly, spheroid cultures were carried out with 1 g Cytodex 3 microcarrier plus 2×10?, 4×10? and ?×10? viable hepatocytes per flask. The numbers of hepatocytes per microcarrier were approximately 666.7, 133.3 and 26.7, respectively. The control group consisted of a spheroid culture of 4×10? hepatocytes without any microcarrier. According to the primary experimental results, spheroid cultures with 1×10? of hepatocytes plus 1 g, 2 g and 3 g of the Cytodex 3 microcarrier were performed. The numbers of hepatocytes per microcarrier were approximately 33.3, 16.7 and 11.1, respectively. The control group consisted of a spheroid culture of 1×10? hepatocytes. The cell viabilities were assayed using a Cell Counting Kit-8; with the albumin production assayed using ELISA.
Results: According to the primary experiment, the group consisting of 26.7 hepatocytes per microcarrier showed the highest viability (P＜0.01). However, there was no statistical difference in the albumin production between the groups (P=0.744). The second Experiment showed the groups consisting of 11.1 and 16.7 hepatocytes per microcarrier had higher viabilities than the other hepatocyte and control groups (P＜0.01). The albumin production was similar for each group (P=0.187).
Conclusion: With respect to their application to a bioartificial liver, about 130 hepatocytes per microcarrier was appeared to be good for the mass cultivation of a hepatocytes spheroid culture using the Cytodex 3 microcarrier.
Methods: Firstly, spheroid cultures were carried out with 1 g Cytodex 3 microcarrier plus 2×10?, 4×10? and ?×10? viable hepatocytes per flask. The numbers of hepatocytes per microcarrier were approximately 666.7, 133.3 and 26.7, respectively. The control group consisted of a spheroid culture of 4×10? hepatocytes without any microcarrier. According to the primary experimental results, spheroid cultures with 1×10? of hepatocytes plus 1 g, 2 g and 3 g of the Cytodex 3 microcarrier were performed. The numbers of hepatocytes per microcarrier were approximately 33.3, 16.7 and 11.1, respectively. The control group consisted of a spheroid culture of 1×10? hepatocytes. The cell viabilities were assayed using a Cell Counting Kit-8; with the albumin production assayed using ELISA.
Results: According to the primary experiment, the group consisting of 26.7 hepatocytes per microcarrier showed the highest viability (P＜0.01). However, there was no statistical difference in the albumin production between the groups (P=0.744). The second Experiment showed the groups consisting of 11.1 and 16.7 hepatocytes per microcarrier had higher viabilities than the other hepatocyte and control groups (P＜0.01). The albumin production was similar for each group (P=0.187).
Conclusion: With respect to their application to a bioartificial liver, about 130 hepatocytes per microcarrier was appeared to be good for the mass cultivation of a hepatocytes spheroid culture using the Cytodex 3 microcarrier.
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REFERENCES
참고문헌 (20)
참고문헌 신청
1. [학술지(정기간행물)] - Rozga J / 1993 / Development of a bioartificial liver: properties and function of a hollow-fiber module inoculated with liver cells / Hepatology 17 : 258 ~ 265
2. [학술지(정기간행물)] - Riordan SM / 1999 / Extracorporeal support and hepatocyte transplantation in acute liver failure and cirrhosis / J Gastroenterol Hepatol 14 : 757 ~ 770
3. [학술지(정기간행물)] - Sussman NL / 1992 / Reversal of fulminant hepatic failure using an extracorporeal liver assist device / Hepatology 16 : 60 ~ 65
4. [학술지(정기간행물)] - Kim BK / 2000 / Liver disease during the first post-transplant year in bone marrow transplantation recipients: retrospective study / Bone Marrow Transplant 26 : 193 ~ 197
5. [학술지(정기간행물)] - Landry J / 1985 / Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities / J Cell Biol 101 : 914 ~ 923
2. [학술지(정기간행물)] - Riordan SM / 1999 / Extracorporeal support and hepatocyte transplantation in acute liver failure and cirrhosis / J Gastroenterol Hepatol 14 : 757 ~ 770
3. [학술지(정기간행물)] - Sussman NL / 1992 / Reversal of fulminant hepatic failure using an extracorporeal liver assist device / Hepatology 16 : 60 ~ 65
4. [학술지(정기간행물)] - Kim BK / 2000 / Liver disease during the first post-transplant year in bone marrow transplantation recipients: retrospective study / Bone Marrow Transplant 26 : 193 ~ 197
5. [학술지(정기간행물)] - Landry J / 1985 / Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities / J Cell Biol 101 : 914 ~ 923
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