지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.87 No.2
- 발행연도
- 2014.7
- 수록면
- 53 - 60 (8page)
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초록· 키워드
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Purpose: To determine the optimum culture conditions by investigating isolated rat hepatocytes cultured in medium containing different glucose concentrations.
Methods: Hepatocytes were isolated from rats using a two-step perfusion technique and divided into the following two groups cultured in medium containing different glucose concentrations: (1) low-glucose group and (2) high-glucose group. Total cell count and viability of cultured rat hepatocytes and liver function parameters (i.e., concentrations of albumin, ammonia, and urea in the culture medium) were measured. The morphology of cultured rat hepatocytes was examined by staining with hematoxylin and eosin, and albumin receptor expression was confirmed by immunofluorescence.
Results: Total cell count and viability showed smaller increases in the low-glucose group than the high-glucose group, although the difference was not statistically significant (P = 0.112 and P = 0.147, respectively). The levels of albumin (P = 0.943), ammonia (P = 0.744), and urea (P = 0.709) were not significantly different between the two groups. In both groups, the function of cultured hepatocytes decreased significantly over time. The morphology of hepatocytes was well maintained in both groups at 3 days. On day 7, the cytoplasm was transformed into a spindle shape. On day 10, these changes were exaggerated, and were more prominent in the high-glucose group.
Conclusion: Morphological assessment indicated that low-glucose culture medium is better than high-glucose culture medium for culturing of hepatocytes, although there was not significantly different in functional assessment. The cultured hepatocytes with low-glucose culture medium could be maintained for 7 days.
Methods: Hepatocytes were isolated from rats using a two-step perfusion technique and divided into the following two groups cultured in medium containing different glucose concentrations: (1) low-glucose group and (2) high-glucose group. Total cell count and viability of cultured rat hepatocytes and liver function parameters (i.e., concentrations of albumin, ammonia, and urea in the culture medium) were measured. The morphology of cultured rat hepatocytes was examined by staining with hematoxylin and eosin, and albumin receptor expression was confirmed by immunofluorescence.
Results: Total cell count and viability showed smaller increases in the low-glucose group than the high-glucose group, although the difference was not statistically significant (P = 0.112 and P = 0.147, respectively). The levels of albumin (P = 0.943), ammonia (P = 0.744), and urea (P = 0.709) were not significantly different between the two groups. In both groups, the function of cultured hepatocytes decreased significantly over time. The morphology of hepatocytes was well maintained in both groups at 3 days. On day 7, the cytoplasm was transformed into a spindle shape. On day 10, these changes were exaggerated, and were more prominent in the high-glucose group.
Conclusion: Morphological assessment indicated that low-glucose culture medium is better than high-glucose culture medium for culturing of hepatocytes, although there was not significantly different in functional assessment. The cultured hepatocytes with low-glucose culture medium could be maintained for 7 days.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - Gotthardt D. / 2007 / Fulminant hepatic failure: etiology and indications for liver transplantation / Nephrol Dial Transplant 22 (Suppl 8) : viii5 ~ viii8
2. [학술지(정기간행물)] - Kjaergard LL. / 2003 / Artificial and bioartificial support systems for acute and acute-on-chronic liver failure : a systematic review / JAMA 289 : 217 ~ 222
3. [학술지(정기간행물)] - Demetriou AA. / 2004 / Prospective, randomized, multicenter, controlled trial of a bioartificial liver in treating acute liver failure / Ann Surg 239 : 660 ~ 667
4. [학술지(정기간행물)] - Gill RQ. / 2001 / Acute liver failure / J Clin Gastroenterol 33 : 191 ~ 198
5. [학술지(정기간행물)] - Dhawan A. / 2010 / Human hepatocyte transplantation : current experience and future challenges / Nat Rev Gastroenterol Hepatol 7 : 288 ~ 298
2. [학술지(정기간행물)] - Kjaergard LL. / 2003 / Artificial and bioartificial support systems for acute and acute-on-chronic liver failure : a systematic review / JAMA 289 : 217 ~ 222
3. [학술지(정기간행물)] - Demetriou AA. / 2004 / Prospective, randomized, multicenter, controlled trial of a bioartificial liver in treating acute liver failure / Ann Surg 239 : 660 ~ 667
4. [학술지(정기간행물)] - Gill RQ. / 2001 / Acute liver failure / J Clin Gastroenterol 33 : 191 ~ 198
5. [학술지(정기간행물)] - Dhawan A. / 2010 / Human hepatocyte transplantation : current experience and future challenges / Nat Rev Gastroenterol Hepatol 7 : 288 ~ 298
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UCI(KEPA) : I410-ECN-0101-2015-510-002473323