지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.2
- 수록면
- 18 - 26 (9page)
- DOI
- 10.15188/kjopp.2015.02.29.1.18
이용수
초록· 키워드
오류제보하기
Akebiae Caulis is a galenical originated from Akebia quinata Decaisne species. It is commonly used in the treatment of oposiuria, inflammation, nociceptive and fever. Here, we investigated the effect of Akebiae Caulis extract (ACE) to protect hepatocyte against the malfunction of mitochondria and apoptosis. Arachidonic acid (AA)+iron promoted excessive reactive oxygen species (ROS) production and exerted a deleterious effect on mitochondria. Treatment with ACE protected hepatocytes from AA+iron-induced cytotoxicity, as shown by alterations in the protein levels related with apoptosis such as poly(ADP-ribose) polymerase, pro-caspase 3, Bcl-XL and Bcl-2. Moreover, AA+iron-induced H₂O₂ production, GSH depletion and mitochondrial dysfunction were alleviated by ACE pretreatment. As a potential molecular mechanism for the ACE-mediated cytoprotection, phosphorylation of AMP-activated protein kinase (AMPK), a key regulator in determining cell survival or death, was increased by ACE. Moreover, ACE treatment enhanced inactive phosphorylation of glycogen synthase kinase-3β (GSK3β), downstream substrate kinase of AMPK. More importantly, ACE prevented a decrease in the GSK3β phosphorylation derived by AA+iron, which might contribute to mitohondiral protection and cell survival. To further identify essential compounds in Akebiae Caulis for the protection of AA+iron-mediated cytotoxicity, we found that betulin in combination with hederagenin protected from AA+iron-induced mitochondrial dysfunction. Betulin+hederagenin treatment also increased inactive phosphorylation of GSK3β in common with ACE. These results suggest that ACE protected hepatocytes against oxidative stress and mitochondrial dysfunction, which is mediated with inactive GSK3β phosphorylation downstream of AMPK.
목차
서론
재료 및 방법
결과
고찰
결론
References
참고문헌 (39)
참고문헌 신청
1. [학술지(정기간행물)] - 김연숙 / 2011 / 돼지감자잎 추출물의 항산화 활성 및 간세포 보호 효과 / 한국식품영양과학회지 40 (11) : 1525 ~ 1531
2. [학술지(정기간행물)] - 오정민 / 2012 / 인간 간암 세포주인 SK-Hep-1에서 Stearic Acid에 의한 산화적 스트레스 및 항산화효소의 발현변화 평가 / 약 학 회 지 56 (1) : 14 ~ 19
3. [학술지(정기간행물)] - 양정민 / 2008 / HeLa cell에서 山藥의 항산화 작용을 통한 세포보호효과에 대한 연구 / 대한한방부인과학회지 21 (2) : 97 ~ 107
4. [학술지(정기간행물)] - 이소연 / 2011 / 산화스트레스가 유도된 인체 간암세포 (HepG2)에서 Sulforaphane과 Diallyl Sulfide, Capsaicin, Gingerol의 항산화효과 비교연구 / Journal of Nutrition and Health 44 (6) : 488 ~ 497
5. [학술지(정기간행물)] - 장주리 / 2011 / 도라지 부탄올 추출물의 항산화 및 nitric oxide 생성 저해 효과 / 한국식품저장유통학회지 18 (1) : 65 ~ 71
2. [학술지(정기간행물)] - 오정민 / 2012 / 인간 간암 세포주인 SK-Hep-1에서 Stearic Acid에 의한 산화적 스트레스 및 항산화효소의 발현변화 평가 / 약 학 회 지 56 (1) : 14 ~ 19
3. [학술지(정기간행물)] - 양정민 / 2008 / HeLa cell에서 山藥의 항산화 작용을 통한 세포보호효과에 대한 연구 / 대한한방부인과학회지 21 (2) : 97 ~ 107
4. [학술지(정기간행물)] - 이소연 / 2011 / 산화스트레스가 유도된 인체 간암세포 (HepG2)에서 Sulforaphane과 Diallyl Sulfide, Capsaicin, Gingerol의 항산화효과 비교연구 / Journal of Nutrition and Health 44 (6) : 488 ~ 497
5. [학술지(정기간행물)] - 장주리 / 2011 / 도라지 부탄올 추출물의 항산화 및 nitric oxide 생성 저해 효과 / 한국식품저장유통학회지 18 (1) : 65 ~ 71
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
AMPK 활성화를 통한 목통의 항산화 효과
동의생리병리학회지
2015 .01
-
삼릉(三稜) 추출물의 간세포 보호 및 미토콘드리아 보호 효과
대한한의학방제학회지
2015 .01
-
夏枯草의 간세포에서 항산화 및 미토콘드리아 보호효과
동의생리병리학회지
2016 .02
-
하고초(夏枯草)의 간세포에서 항산화 및 미토콘드리아 보호효과
동의생리병리학회지
2016 .01
-
Exposure to Adverse Childhood Experiences (ACEs) and Stress among the Community-based Urban Pediatric Population in Korea
Journal of Korean Medical Science
2020 .01
-
The angiotensin-converting enzyme 2 (ACE2) receptor in the prevention and treatment of COVID-19 are distinctly different paradigms
Clinical Hypertension
2020 .01
-
Comparing the safety of harmonic ACE and ACE+ around the recurrent laryngeal nerve in swine models
Annals of Surgical Treatment and Research
2018 .06
-
코로나바이러스19 감염증과 안지오텐신전환효소2
대한내과학회지
2020 .08
-
합환피 추출물의 지방세포 분화 억제 효과
동의생리병리학회지
2016 .12
-
Different expression of GSK3β and pS9GSK3β depending on phenotype of cervical cancer: possible association of GSK3β with squamous cell carcinoma and pS9GSK3β with adenocarcinoma
Obstetrics & Gynecology Science
2019 .01
-
Role of AMP-Activated Protein Kinase (AMPK) in Smoking-Induced Lung Inflammation and Emphysema
Tuberculosis and Respiratory Diseases
2015 .01
-
The Thai Version of the Addenbrooke’s Cognitive Examination III
PSYCHIATRY INVESTIGATION
2016 .01
-
Lack of Influence of the ACE1 Gene I/D Polymorphism on the Formation and Growth of Benign Uterine Leiomyoma in Turkish Patients
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
The Impact of Maternal Adverse Childhood Experiences on Offspring’s Internalizing and Externalizing Problems
PSYCHIATRY INVESTIGATION
2021 .11
-
Reserpine treatment activates AMP activated protein kinase (AMPK)
Natural Product Sciences
2017 .09
-
Artemisia annua extract ameliorates high-fat diet-induced fatty liver by activating AMPK
Journal of Biomedical and Translational Research
2020 .01
-
The Characteristics and Survival Outcomes in Patients Aged 70 Years and Older with Nasopharyngeal Carcinoma in the Intensity-Modulated Radiotherapy Era
Cancer Research and Treatment
2019 .01
-
Anti-adipogenic effect of the flavonoids through the activation of AMPK in palmitate (PA)-treated HepG2 cells
Journal of Veterinary Science
2022 .01
-
Effect of iron overload with ascorbic acid on experimental coloncarcinogenesis in mice
Journal of Biomedical and Translational Research
2017 .12
-
L-Ascorbic Acid Induces Apoptosis in Human Laryngeal Epidermoid Hep-2 Cells by Modulating the NF-κB/MAPK/Akt Signaling Pathway
International Journal of Oral Biology
2020 .01
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2016-519-001127050