지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.2
- 수록면
- 77 - 83 (7page)
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초록· 키워드
오류제보하기
Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed to screen filarial parasites in their vectors by single and multiplex PCR and evaluate the usefulness of multiplex PCR as a rapid xenomonitoring and simultaneous differentiation tool, in area where 3 filarial parasites are coexisting. Female mosquitoes were collected from 7 localities in Assiut Governorate, were microscopically identified and divided into pools according to their species and collection site. Detection of W. bancrofti, D. immitis, and D. repens using single PCR was reached followed by multiplex PCR. Usefulness of multiplex PCR was evaluated by testing mosquito pools to know which genera and species are used by filarial parasites as a vector. An overall estimated rate of infection (ERI) in mosquitoes was 0.6%; the highest was Culex spp. (0.47%). W. bancrofti, D. immitis, and D. repens could be simultaneously and differentially detected in infected vectors by using multiplex PCR. Out of 100 mosquito pools, 8 were positive for W. bancrofti (ERI of 0.33%) and 3 pools each were positive for D. immitis and D. repens (ERI 0.12%). The technique showed 100% sensitivity and 98% specificity. El-Nikhila, El-Matiaa villages, and Sahel Seleem district in Assiut Governorate, Egypt are still endemic foci for filarial parasites. Multiplex PCR offers a reliable procedure for molecular xenomonitoring of filariasis within their respective vectors in endemic areas. Therefore, it is recommended for evaluation of mosquito infection after lymphatic filariasis eradication programs.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (34)
참고문헌 신청
Hopkins DR / 2013 / Disease eradication / N Engl J Med 368:54~63
Ramzy RM / 2005 / Defining the cost of the Egyptian lymphatic filariasis elimination programme / Filaria J 4:7
World Health Organization / Annual country reports
Addiss DG / 2005 / Global elimination of lymphatic filariasis: origins, progress and challenges / Indian J Urol 7:12~17
Genchi C / 2011 / Dirofilarial infections in Europe / Vector Borne Zoon Dis 11:1307~1317
Ramzy RM / 2005 / Defining the cost of the Egyptian lymphatic filariasis elimination programme / Filaria J 4:7
World Health Organization / Annual country reports
Addiss DG / 2005 / Global elimination of lymphatic filariasis: origins, progress and challenges / Indian J Urol 7:12~17
Genchi C / 2011 / Dirofilarial infections in Europe / Vector Borne Zoon Dis 11:1307~1317
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UCI(KEPA) : I410-ECN-0101-2016-513-001133949