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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Preventive Nutrition and Food Science Preventive Nutrition and Food Science Vol.20 No.2
- 발행연도
- 2015.6
- 수록면
- 119 - 125 (7page)
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초록· 키워드
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The biologically active compounds raphasatin and sulforaphene are formed during the hydrolysis of radishes by an endogenous myrosinase. Raphasatin is very unstable, and it is generated and simultaneously degraded to less active compounds during hydrolysis in aqueous media. This study determined the hydrolysis conditions to maximize the formation of raphasatin and sulforaphene by an endogenous myrosinase and minimize their degradation during the hydrolysis of radish roots. The reaction parameters, such as the reaction medium, reaction time, type of mixing, and reaction temperature were optimized. A stability test for raphasatin and sulforaphene was also performed during storage of the hydrolyzed products at 25℃ for 10 days. The formation and breakdown of raphasatin and sulforaphene in radish roots by endogenous enzymolysis was strongly influenced by the reaction medium, reaction time, and type of mixing. The production and stabilization of raphasatin in radishes was efficient in water and dichloromethane with shaking for 15 min at 25℃. For sulforaphene, the favorable condition was water as the reaction medium without shaking for 10 min at 25℃. The maximum yields of raphasatin and sulforaphene were achieved in a concurrent hydrolysis reaction without shaking in water for 10 min and then with shaking in dichloromethane for 15 min at 25℃. Under these conditions, the yields of raphasatin and sulforaphene were maximized at 12.89 and 1.93 μmol/g of dry radish, respectively. The stabilities of raphasatin and sulforaphene in the hydrolyzed products were 56.4% and 86.5% after 10 days of storage in water and dichloromethane at 25℃.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (19)
참고문헌 신청
[학술지(정기간행물)] - Gutiérrez RMP / 2004 / Raphanus sativus(radish) : their chemistry and biology / Sci World J 4 : 811 ~ 837
[학술지(정기간행물)] - Hanlon PR / 2011 / Phytochemical composition and biological activity of 8 varieties of radish (Raphanus sativus L.) sprouts and mature taproots / J Food Sci 76 : C185 ~ C192
[학술지(정기간행물)] - Papi A / 2008 / Cytotoxic and antioxidant activity of 4-methylthio-3-butenyl isothiocyanate from Raphanus sativus L. (kaiware daikon)sprouts / J Agric Food Chem 56 : 875 ~ 883
[학술지(정기간행물)] - Yuan G / 2010 / Effect of salt stress on phenolic compounds, glucosinolates, myrosinase and antioxidant activity in radish sprouts / Food Chem 121 : 1014 ~ 1019
[학술지(정기간행물)] - Abdull Razis AF / 2012 / 4-Methylsulfanyl-3-butenyl isothiocyanate derived from glucoraphasatin is a potent inducer of rat hepatic phase II enzymes and a potential chemopreventive agent / Arch Toxicol 86 : 183 ~ 194
[학술지(정기간행물)] - Hanlon PR / 2011 / Phytochemical composition and biological activity of 8 varieties of radish (Raphanus sativus L.) sprouts and mature taproots / J Food Sci 76 : C185 ~ C192
[학술지(정기간행물)] - Papi A / 2008 / Cytotoxic and antioxidant activity of 4-methylthio-3-butenyl isothiocyanate from Raphanus sativus L. (kaiware daikon)sprouts / J Agric Food Chem 56 : 875 ~ 883
[학술지(정기간행물)] - Yuan G / 2010 / Effect of salt stress on phenolic compounds, glucosinolates, myrosinase and antioxidant activity in radish sprouts / Food Chem 121 : 1014 ~ 1019
[학술지(정기간행물)] - Abdull Razis AF / 2012 / 4-Methylsulfanyl-3-butenyl isothiocyanate derived from glucoraphasatin is a potent inducer of rat hepatic phase II enzymes and a potential chemopreventive agent / Arch Toxicol 86 : 183 ~ 194
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UCI(KEPA) : I410-ECN-0101-2016-594-001674253