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논문 기본 정보

자료유형
학술저널
저자정보
Ji Won Son (The Catholic University Seoul) Ho Yun Choi (The Catholic University Seoul) Han Na Lee (The Catholic University Seoul) Min Koo Seo (The Catholic University Seoul) Suk Kyeong Lee (The Catholic University Seoul)
저널정보
대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY Journal of Bacteriology and Virology 제44권 제1호
발행연도
2014.3
수록면
84 - 94 (11page)

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Epstein-Barr virus (EBV)-encoded small non-coding RNAs (EBERs) are abundantly expressed in various EBVassociated malignancies, and play critical roles in cell proliferation, tumorigenesis, and apoptosis resistance. However, the mechanism how EBERs regulate cell function awaits further clarification. In this study, we investigated the effect of EBERs on the expression of cellular microRNA (miRNA) and mRNA expression. To test the effect of EBERs while unaffected by other EBV genes, we used EBERs-deleted recombinant EBV infected Burkitt"s lymphoma cell line (Akata(+)EBERs(-)) as well as EBV-infected (Akata(+)) and EBV uninfected (Akata(-)) cell lines. They all have the same genetic backgrounds. First, 15 different cellular miRNAs which have reverse complementary sequences to EBERs and have reported targets were selected by bioinformatics analysis. When RT-PCR was carried out for the 16 miRNAs using RNAs from Akata(+), Akata(-), and Akata(+)EBERs(-) cells, hsa-miR-7-5p was the only one showing downregulated expression in Akata(+) than in Akata(-) and Akata(+)EBERs(-) cells. Bioinformatics and mRNA microarray analyses for Akata(+), Akata(-), and Akata(+)EBERs(-) cell lines were then carried out to predict putative targets of hsa-miR-7-5p. Among the 6 predicted targets of hsa-miR-7-5p, only low density lipoprotein receptor-related protein 6 (LRP6) was up-regulated in EBERs-expressing cells when tested by RT-PCR and Western blot. However, luciferase reporter assay showed that the 3"-UTR of LRP6 was not directly targeted by hsa-miR-7-5p. Our data suggest that both hsa-miR-7-5p and LRP6 are regulated by EBERs in Akata cells, and these genes may partly mediate the tumorigenic function of EBERs in Burkitt"s lymphoma.
#Epstein-Barr virus #EBERs #microRNA #Burkitt's lymphoma cell #LRP6 #hsa-miR-7-5p

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

참고문헌 (39)

참고문헌 신청
1. [학술지(정기간행물)] - Thompson MP. / 2004 / Epstein-Barr virus and cancer / Clin Cancer Res 10 : 803 ~ 821 google schola 2. [단행본] - Kieff E / 2007 / Fields virology / Raven Press : 2603 ~ 2654 google schola 3. [학술지(정기간행물)] - Rosa MD. / 1981 / Striking similarities are exhibited by two small Epstein-Barr virusencoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII / Mol Cell Biol 1 : 785 ~ 796 google schola 4. [학술지(정기간행물)] - Rymo L. / 1979 / Identification of transcribed regions of Epstein-Barr virus DNA in Burkitt lymphoma-derived cells / J Virol 32 : 8 ~ 18 google schola 5. [학술지(정기간행물)] - Arrand JR. / 1989 / Two families of sequences in the small RNA-encoding region of Epstein-Barr virus(EBV)correlate with EBV types A and B / J Virol 63 : 983 ~ 986 google schola

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