지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Transgenic animals have been widely used for developmental biology studies, as disease models, and even in industry such as transgenic bioreactor animals. For transgenic birds, quail has the great advantages of small body size, short generation time, and frequent egg production. To date, retroviral or lentiviral transduction has been used to generate transgenic quail for various purposes. However, the efficiency of transgenic offspring production with these methods is relatively low and viral vector usage has safety issues. Unfortunately, non-viral transgenesis has not been established in quail due to a deficiency of stem cell and germ cell culture systems. In this study, we established a direct in ovo lipofection method that could be used to create transgenic quail without germline-competent cells or viruses. To optimize the injection stage during embryo development, the liposome complex (containing piggyBacCMV-GFP and transposase plasmids) was introduced into an embryonic blood vessel at 50 hr, 55 hr or 60 hr. GFP expression was detected in various tissues (heart, kidney, liver and stomach) on day 12 of incubation under a fluorescence microscope. Additionally, GFP-positive cells were detected in the recipient embryonic gonads. In conclusion, the direct in ovo lipofection method with the piggyBac transposon could be an efficient and useful tool for generating transgenic quail.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)] - Agate RJ / 2009 / Transgenic songbirds offer an opportunity to develop a genetic model for vocal learning / Proc Natl Acad Sci 106 : 17963 ~ 17967
[학술지(정기간행물)] - Carsience RS / 1993 / Germline chimeric chickens from dispersed donor blastodermal cells and compromised recipient embryos / Development 117 : 669 ~ 675
[학술지(정기간행물)] - Choi JW / 2010 / Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells / PLoS One 5 : e12968 ~
[학술지(정기간행물)] - Harvey AJ / 2002 / Expression of exogenous protein in the egg white of transgenic chickens / Nat Biotechnol 20 : 396 ~ 399
[학술지(정기간행물)] - Kwon SC / 2010 / Production of biofunctional recombinant human interleukin 1 receptor antagonist (rhIL1RN) from transgenic quail egg white / Biol Reprod 82 : 1057 ~ 1064
[학술지(정기간행물)] - Carsience RS / 1993 / Germline chimeric chickens from dispersed donor blastodermal cells and compromised recipient embryos / Development 117 : 669 ~ 675
[학술지(정기간행물)] - Choi JW / 2010 / Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells / PLoS One 5 : e12968 ~
[학술지(정기간행물)] - Harvey AJ / 2002 / Expression of exogenous protein in the egg white of transgenic chickens / Nat Biotechnol 20 : 396 ~ 399
[학술지(정기간행물)] - Kwon SC / 2010 / Production of biofunctional recombinant human interleukin 1 receptor antagonist (rhIL1RN) from transgenic quail egg white / Biol Reprod 82 : 1057 ~ 1064
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UCI(KEPA) : I410-ECN-0101-2016-527-001973730