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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 183 - 193 (11page)
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Several transgenic marine medaka Oryzias dancena strains harboring a green fluorescent protein (GFP) reporter construct regulated by an endogenous β-actin promoter were established and their expression characteristics in relation to transgene copy numbers were examined in 21 transgene genotypes. Most of the transgenic strains displayed transgene insertion patterns typical of microinjection-mediated introduction of foreign DNA into fish embryos, characterized by the random integration of multiple transgene copies (ranging from 1 – 282 copies per cell), often accompanied by the formation of concatemer(s), as assessed by genomic Southern blot hybridization analysis and qPCR. Transgenic strains showed ubiquitous and continued temporal and spatial expression patterns of the transgenic GFP during most of their life cycle, from the embryonic stage to adulthood, enabling assessment of the expression pattern of the endogenous β-actin gene. However, a comparative evaluation of transgene copy numbers and expression levels showed that copy number-dependent expression, the stability of the ubiquitous distribution and expression efficiency per transgene copy varied among the transgenic strains. Fluorescence expression levels were positively correlated with absolute transgene copy numbers, whereas the expression efficiency per transgene copy was inversely related to the number of transgene integrant copies. Data from this study will guide the selection of potentially desirable transgenic strains with ubiquitous expression of a fluorescent transgene, not only in this marine medaka species but also in other related model fish species.
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참고문헌 (23)
참고문헌 신청
Baup D / 2010 / Variegation and silencing in a lentiviral-based murine transgenic model / Transgenic Res 19 : 399 ~ 414
Burket CT / 2008 / Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters / Transgenic Res 17 : 265 ~ 279
Chen TT / 1996 / Transgenic fish and its application in basic and applied research / Biotechnol Annu Rev 2 : 205 ~ 236
Cho YS / 2011 / Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of t
Cho YS / 2013 / Characterization of estrogenresponsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogeno
Burket CT / 2008 / Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters / Transgenic Res 17 : 265 ~ 279
Chen TT / 1996 / Transgenic fish and its application in basic and applied research / Biotechnol Annu Rev 2 : 205 ~ 236
Cho YS / 2011 / Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of t
Cho YS / 2013 / Characterization of estrogenresponsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogeno
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