지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
The thermal properties of ramie leaf β-amylase (RBA) were examined to develop a novel process for enzyme purification. The thermostability of RBA extract prepared from ramie leaf powder was examined at various temperatures. RBA activity decreased slightly, whereas other carbohydrate-active enzymes, such as D-enzyme, were rapidly inactivated during 30 min incubation at 60℃. When the heat-treated extract was incubated with various substrates, maltose was produced exclusively as the major product, whereas the untreated crude extract produced maltose and other maltooligosaccharides. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, fewer protein bands were observed for the heat-treated extract than the untreated extract, indicating that the thermostable RBA was partially purified and other thermolabile enzymes were eliminated. Thus, the treatment of the RBA extract at 60℃ for 30 min resulted in 5.4-fold purification with a recovery yield of 90%.
목차
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (30)
참고문헌 신청
[학술지(정기간행물)] - Kitahata S / 1991 / Mechanism of maltal hydration catalyzed by beta-amylase: role of protein structure in controlling the steric outcome of reactions catalyzed by a glycosylase / Biochemistry 30 : 6769 ~ 6775
[학술지(정기간행물)] - Ruzanski C / 2013 / A bacterial glucanotransferase can replace the complex maltose metabolism required for starch to sucrose conversion in leaves at night / J. Biol. Chem 288 : 28581 ~ 28598
[학술지(정기간행물)] - Takaha T / 1993 / Disproportionating enzyme (4-α-Glucanotransferase; EC 2.4.1.25) of potato: purification, molecular cloning, and potential role in starch metabolism / J. Biol. Chem 268 : 1391 ~ 1396
[학술지(정기간행물)] - Cenci U / 2014 / Transition from glycogen to starch metabolism in archaeplastida / Trends Plant Sci 19 : 18 ~ 28
[학술지(정기간행물)] - Daba T / 2012 / Characterization and solvent engineering of wheat β-amylase for enhancing its activity and stability / Enzyme Microb. Technol 51 : 245 ~ 251
[학술지(정기간행물)] - Ruzanski C / 2013 / A bacterial glucanotransferase can replace the complex maltose metabolism required for starch to sucrose conversion in leaves at night / J. Biol. Chem 288 : 28581 ~ 28598
[학술지(정기간행물)] - Takaha T / 1993 / Disproportionating enzyme (4-α-Glucanotransferase; EC 2.4.1.25) of potato: purification, molecular cloning, and potential role in starch metabolism / J. Biol. Chem 268 : 1391 ~ 1396
[학술지(정기간행물)] - Cenci U / 2014 / Transition from glycogen to starch metabolism in archaeplastida / Trends Plant Sci 19 : 18 ~ 28
[학술지(정기간행물)] - Daba T / 2012 / Characterization and solvent engineering of wheat β-amylase for enhancing its activity and stability / Enzyme Microb. Technol 51 : 245 ~ 251
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UCI(KEPA) : I410-ECN-0101-2017-594-001870513