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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 366 - 374 (9page)
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초록· 키워드
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Background: In this study, we screened and identified an endophyte JG09 having strong biocatalytic activity for ginsenosides from Platycodon grandiflorum, converted ginseng total saponins and ginsenoside monomers, determined the source of minor ginsenosides and the transformation pathways, and calculated the maximum production of minor ginsenosides for the conversion of ginsenoside Rb1 to assess the transformation activity of endophyte JG09.
Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis.
Results: A total of 32 β-glucosidase-producing endophytes were screened out among the isolated 69 endophytes from P. grandiflorum. An endophyte bacteria JG09 identified as Luteibacter sp. effectively converted protopanaxadiol-type ginsenosides Rb1, Rb2, Rc, Rd into minor ginsenosides F2 and C-K, and converted protopanaxatriol-type ginsenoside Rg1 into minor ginsenoside Rh1. The transformation pathways of major ginsenosides by endophyte JG09 were as follows: Rb1→Rd→F2→C-K; Rb2→C-O→C-Y→C-K; Rc→C-Mc1→C-Mc→C-K; Rg1→Rh1. The maximum production rate of ginsenosides F2 and C-K reached 94.53% and 66.34%, respectively.
Conclusion: This is the first report about conversion of major ginsenosides into minor ginsenosides by fermentation with P. grandiflorum endophytes. The results of the study indicate endophyte JG09 would be a potential microbial source for obtaining minor ginsenosides.
Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis.
Results: A total of 32 β-glucosidase-producing endophytes were screened out among the isolated 69 endophytes from P. grandiflorum. An endophyte bacteria JG09 identified as Luteibacter sp. effectively converted protopanaxadiol-type ginsenosides Rb1, Rb2, Rc, Rd into minor ginsenosides F2 and C-K, and converted protopanaxatriol-type ginsenoside Rg1 into minor ginsenoside Rh1. The transformation pathways of major ginsenosides by endophyte JG09 were as follows: Rb1→Rd→F2→C-K; Rb2→C-O→C-Y→C-K; Rc→C-Mc1→C-Mc→C-K; Rg1→Rh1. The maximum production rate of ginsenosides F2 and C-K reached 94.53% and 66.34%, respectively.
Conclusion: This is the first report about conversion of major ginsenosides into minor ginsenosides by fermentation with P. grandiflorum endophytes. The results of the study indicate endophyte JG09 would be a potential microbial source for obtaining minor ginsenosides.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (33)
참고문헌 신청
[학술지(정기간행물)] - Qi LW / 2011 / Isolation and analysis of ginseng: advances and challenges / Nat Prod Rep 28 : 467 ~ 495
[학술지(정기간행물)] - Jia L / 2009 / Current evaluation of the millennium phytomedicine-ginseng (I):etymology, pharmacognosy, phytochemistry, market and regulations / Curr Med Chem 16 : 2475 ~ 2484
[학술지(정기간행물)] - Wang L / 2011 / Bioconversion of ginsenosides Rb1, Rb2, Rc and Rd by novel b-glucosidase hydrolyzing outer 3-O glycoside from Sphingomonas sp. 2F2: Cloning, expression, and enzyme characterization / J Biotechnol 156 : 125 ~ 133
[학술지(정기간행물)] - Li W / 2011 / Snailase preparation of ginsenoside M1 from protopanaxadiol-type ginsenoside and their protective effects against CCl4-induced chronic hepatotoxicity in mice / Molecules 16 : 10093 ~ 10103
[학술지(정기간행물)] - Chen Y / 2013 / Anti-cancer effects of ginsenoside compound k on pediatric acute myeloid leukemia cells / Cancer Cell Int 13 : 24 ~
[학술지(정기간행물)] - Jia L / 2009 / Current evaluation of the millennium phytomedicine-ginseng (I):etymology, pharmacognosy, phytochemistry, market and regulations / Curr Med Chem 16 : 2475 ~ 2484
[학술지(정기간행물)] - Wang L / 2011 / Bioconversion of ginsenosides Rb1, Rb2, Rc and Rd by novel b-glucosidase hydrolyzing outer 3-O glycoside from Sphingomonas sp. 2F2: Cloning, expression, and enzyme characterization / J Biotechnol 156 : 125 ~ 133
[학술지(정기간행물)] - Li W / 2011 / Snailase preparation of ginsenoside M1 from protopanaxadiol-type ginsenoside and their protective effects against CCl4-induced chronic hepatotoxicity in mice / Molecules 16 : 10093 ~ 10103
[학술지(정기간행물)] - Chen Y / 2013 / Anti-cancer effects of ginsenoside compound k on pediatric acute myeloid leukemia cells / Cancer Cell Int 13 : 24 ~
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UCI(KEPA) : I410-ECN-0101-2018-524-001598496