지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 151 - 159 (9page)
이용수
초록· 키워드
오류제보하기
Background: Ginsenoside-Rg3, the pharmacologically active component of red ginseng, has been found to inhibit tumor growth, invasion, metastasis, and angiogenesis in various cancer models. Previously, we found that 20(R)-ginsenoside-Rg3 (Rg3) could inhibit angiogenesis. Since microRNAs (miRNAs) have been shown to affect many biological processes, they might play an important role in ginsenosidemediated angiomodulation.
Methods: In this study, we examined the underlying mechanisms of Rg3-induced angiosuppression through modulating the miRNA expression. In the miRNA-expression profiling analysis, six miRNAs and three miRNAs were found to be up- or down-regulated in vascular-endothelial-growth-factor-induced human-umbilical-vein endothelial cells (HUVECs) after Rg3 treatment, respectively.
Results: A computational prediction suggested that mature hsa-miR-520h (miR-520h) targets ephrin receptor (Eph) B2 and EphB4, and hence, affecting angiogenesis. The up-regulation of miR-520h after Rg3 treatment was validated by quantitative real-time polymerase chain reaction, while the protein expressions of EphB2 and EphB4 were found to decrease, respectively. The mimics and inhibitors of miR-520h were transfected into HUVECs and injected into zebra-fish embryos. The results showed that overexpression of miR-520h could significantly suppress the EphB2 and EphB4 protein expression, proliferation, and tubulogenesis of HUVECs, and the subintestinal-vessel formation of the zebra fish.
Conclusion: These results might provide further information on the mechanism of Rg3-induced angiosuppression and the involvement of miRNAs in angiogenesis.
Methods: In this study, we examined the underlying mechanisms of Rg3-induced angiosuppression through modulating the miRNA expression. In the miRNA-expression profiling analysis, six miRNAs and three miRNAs were found to be up- or down-regulated in vascular-endothelial-growth-factor-induced human-umbilical-vein endothelial cells (HUVECs) after Rg3 treatment, respectively.
Results: A computational prediction suggested that mature hsa-miR-520h (miR-520h) targets ephrin receptor (Eph) B2 and EphB4, and hence, affecting angiogenesis. The up-regulation of miR-520h after Rg3 treatment was validated by quantitative real-time polymerase chain reaction, while the protein expressions of EphB2 and EphB4 were found to decrease, respectively. The mimics and inhibitors of miR-520h were transfected into HUVECs and injected into zebra-fish embryos. The results showed that overexpression of miR-520h could significantly suppress the EphB2 and EphB4 protein expression, proliferation, and tubulogenesis of HUVECs, and the subintestinal-vessel formation of the zebra fish.
Conclusion: These results might provide further information on the mechanism of Rg3-induced angiosuppression and the involvement of miRNAs in angiogenesis.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusion
References
참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] - Rosen ED / 2014 / What we talk about when we talk about fat / Cell 156 : 20 ~ 44
[학술지(정기간행물)] - Weisberg SP / 2003 / Obesity is associated with macrophage accumulation in adipose tissue / J Clin Invest 112 : 1796 ~ 1808
[학술지(정기간행물)] - Siriwardhana N / 2012 / n-3 and n-6 polyunsaturated fatty acids differentially regulate adipose angiotensinogen and other inflammatory adipokines in part via NF-kB-dependent mechanisms / J Nutr Biochem 23 : 1661 ~ 1667
[학술지(정기간행물)] - Barnes PM / 2008 / Complementary and alternative medicine use among adults and children: United States, 2007 / Natl Health Stat Report 12 : 1 ~ 23
[학술지(정기간행물)] - Wilson SA / 2013 / Unraveling the adipocyte inflammomodulatory pathways activated by North American ginseng / Int J Obes (Lond) 37 : 350 ~ 356
[학술지(정기간행물)] - Weisberg SP / 2003 / Obesity is associated with macrophage accumulation in adipose tissue / J Clin Invest 112 : 1796 ~ 1808
[학술지(정기간행물)] - Siriwardhana N / 2012 / n-3 and n-6 polyunsaturated fatty acids differentially regulate adipose angiotensinogen and other inflammatory adipokines in part via NF-kB-dependent mechanisms / J Nutr Biochem 23 : 1661 ~ 1667
[학술지(정기간행물)] - Barnes PM / 2008 / Complementary and alternative medicine use among adults and children: United States, 2007 / Natl Health Stat Report 12 : 1 ~ 23
[학술지(정기간행물)] - Wilson SA / 2013 / Unraveling the adipocyte inflammomodulatory pathways activated by North American ginseng / Int J Obes (Lond) 37 : 350 ~ 356
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UCI(KEPA) : I410-ECN-0101-2018-524-001598229