Pyruvate Protects Giardia Trophozoites from Cysteine-Ascorbate Deprived Medium Induced Cytotoxicity

Dibyendu Raj; Punam Chowdhury; Rituparna Sarkar; Yumiko Saito-Nakano; Keinosuke Okamoto; Shanta Dutta; Tomoyoshi Nozaki; Sandipan Ganguly

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저자정보: Dibyendu Raj (Vivekananda College) Punam Chowdhury (National Institute of Cholera and Enteric Diseases) Rituparna Sarkar (National Institute of Cholera and Enteric Diseases) Yumiko Saito-Nakano (National Institute of Infectious Diseases) Keinosuke Okamoto (Okayama University for Infectious Diseases) Shanta Dutta (National Institute of Cholera and Enteric Diseases) Tomoyoshi Nozaki (The University of Tokyo) Sandipan Ganguly (National Institute of Cholera and Enteric Diseases)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.56 No.1

발행연도: 2018.2

수록면: 1 - 12 (12page)

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Giardia lamblia, an anaerobic, amitochondriate protozoan parasite causes parasitic infection giardiasis in children and young adults. It produces pyruvate, a major metabolic product for its fermentative metabolism. The current study was undertaken to explore the effects of pyruvate as a physiological antioxidant during oxidative stress in Giardia by cysteine-ascorbate deprivation and further investigation upon the hypothesis that oxidative stress due to metabolism was the reason behind the cytotoxicity. We have estimated intracellular reactive oxygen species generation due to cysteine-ascorbate deprivation in Giardia. In the present study, we have examined the effects of extracellular addition of pyruvate, during oxidative stress generated from cysteine-ascorbate deprivation in culture media on DNA damage in Giardia. The intracellular pyruvate concentrations at several time points were measured in the trophozoites during stress. Trophozoites viability under cysteine-ascorbate deprived (CAD) medium in presence and absence of extracellular pyruvate has also been measured. The exogenous addition of a physiologically relevant concentration of pyruvate to trophozoites suspension was shown to attenuate the rate of ROS generation. We have demonstrated that Giardia protects itself from destructive consequences of ROS by maintaining the intracellular pyruvate concentration. Pyruvate recovers Giardia trophozoites from oxidative stress by decreasing the number of DNA breaks that might favor DNA repair.

#Giardia lamblia #oxidative stress #cysteine-ascorbate #antioxidant #pyruvate #reactive oxygen species #cytotoxicity

참고문헌 (29)

참고문헌 신청

Adam RD / 1991 / The biology of Giardia spp / Microbiol Rev 55:706~732

Adam RD / 2001 / Biology of Giardia lamblia / Clin Microbiol Rev 14:447~475

Lane S / 2002 / Current trends in research into the waterborne parasite Giardia / Crit Rev Microbiol 28:123~147

Tak V / 2014 / Molecular Characterization of Giardia intestinalis assemblages from human isolates at a tertiary care center of India / Ind J Med Micro 32:19~25

Farthing MJ / 1997 / The molecular pathogenesis of giardiasis / J Pediatr Gastroenterol Nutr 24:79~88

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