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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 183 - 190 (8page)
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BACKGROUND/OBJECTIVE: This study was designed to investigate how a Portulaca oleracea L. extract (POE) stimulates insulin secretion in INS-1 pancreatic β-cells.
MATERIALS/METHOD: INS-1 pancreatic β-cells were incubated in the presence of various glucose concentrations: 1.1 or 5.6, 16.7 mM glucose. The cells were treated with insulin secretagogues or insulin secretion inhibitor for insulin secretion assay using an insulin ELISA kit. In order to quantify intracellular influx of Ca<SUP>2+</SUP> caused by POE treatment, the effect of POE on intracellular Ca<SUP>2+</SUP> in INS-1 pancreatic β-cells was examined using Fluo-2 AM dye.
RESULTS: POE at 10 to 200 ㎍/mL significantly increased insulin secretion dose-dependently as compared to the control. Experiments at three glucose concentrations (1.1, 5.6, and 16.7 mM) confirmed that POE significantly stimulated insulin secretion on its own as well as in a glucose-dependent manner. POE also exerted synergistic effects on insulin secretion with secretagogues, such as L-alanine, 3-isobutyl-1-methylxanthine, and especially tolbutamide, and at a depolarizing concentration of KCl. The insulin secretion caused by POE was significantly attenuated by treatment with diazoxide, an opener of the K⁺<SUB>ATP</SUB> channel (blocking insulin secretion) and by verapamil (a Ca<SUP>2+</SUP> channel blocker). The insulinotropic effect of POE was not observed under Ca<SUP>2+</SUP>-free conditions in INS-1 pancreatic β-cells. When the cells were preincubated with a Ca<SUP>2+</SUP> fluorescent dye, Fluo-2 (acetoxymethyl ester), the cells treated with POE showed changes in fluorescence in red, green, and blue tones, indicating a significant increase in intracellular Ca<SUP>2+</SUP>, which closely correlated with increases in the levels of insulin secretion.
CONCLUSIONS: These findings indicate that POE stimulates insulin secretion via a K⁺<SUB>ATP</SUB> channel-dependent pathway in INS-1 pancreatic β-cells.
MATERIALS/METHOD: INS-1 pancreatic β-cells were incubated in the presence of various glucose concentrations: 1.1 or 5.6, 16.7 mM glucose. The cells were treated with insulin secretagogues or insulin secretion inhibitor for insulin secretion assay using an insulin ELISA kit. In order to quantify intracellular influx of Ca<SUP>2+</SUP> caused by POE treatment, the effect of POE on intracellular Ca<SUP>2+</SUP> in INS-1 pancreatic β-cells was examined using Fluo-2 AM dye.
RESULTS: POE at 10 to 200 ㎍/mL significantly increased insulin secretion dose-dependently as compared to the control. Experiments at three glucose concentrations (1.1, 5.6, and 16.7 mM) confirmed that POE significantly stimulated insulin secretion on its own as well as in a glucose-dependent manner. POE also exerted synergistic effects on insulin secretion with secretagogues, such as L-alanine, 3-isobutyl-1-methylxanthine, and especially tolbutamide, and at a depolarizing concentration of KCl. The insulin secretion caused by POE was significantly attenuated by treatment with diazoxide, an opener of the K⁺<SUB>ATP</SUB> channel (blocking insulin secretion) and by verapamil (a Ca<SUP>2+</SUP> channel blocker). The insulinotropic effect of POE was not observed under Ca<SUP>2+</SUP>-free conditions in INS-1 pancreatic β-cells. When the cells were preincubated with a Ca<SUP>2+</SUP> fluorescent dye, Fluo-2 (acetoxymethyl ester), the cells treated with POE showed changes in fluorescence in red, green, and blue tones, indicating a significant increase in intracellular Ca<SUP>2+</SUP>, which closely correlated with increases in the levels of insulin secretion.
CONCLUSIONS: These findings indicate that POE stimulates insulin secretion via a K⁺<SUB>ATP</SUB> channel-dependent pathway in INS-1 pancreatic β-cells.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
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UCI(KEPA) : I410-ECN-0101-2018-594-002057327