지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 288 - 297 (10page)
이용수
초록· 키워드
오류제보하기
Background: The incidence of colorectal cancer (CRC) is increasing, with metastasis of newly diagnosed CRC reported in a large proportion of patients. However, the effect of Korean Red Ginseng extracts (KRGE) on epithelial to mesenchymal transition (EMT) in CRC is unknown. Therefore, we examined the mechanisms by which KRGE regulates EMT of CRC in hypoxic conditions.
Methods: Human CRC cell lines HT29 and HCT116 were incubated under hypoxic (1% oxygen) and normoxic (21% oxygen) conditions. Western blot analysis and real-time PCR were used to evaluate the expression of EMT markers in the presence of KRGE. Furthermore, we performed scratched wound healing, transwell migration, and invasion assays to monitor whether KRGE affects migratory and invasive abilities of CRC cells under hypoxic conditions.
Results: KRGE-treated HT29 and HCT116 cells displayed attenuated vascular endothelial growth factor (VEGF) mRNA levels and hypoxia-inducible factor-1a (HIF-1a) protein expression under hypoxic conditions. KRGE repressed Snail, Slug, and Twist mRNA expression and integrin aVb6 protein levels. Furthermore, hypoxia-repressed E-cadherin was restored in KRGE-treated cells; KRGE blocked the invasion and migration of colon cancer cells by repressing NF-kB and ERK1/2 pathways in hypoxia.
Conclusions: KRGE inhibits hypoxia-induced EMT by repressing NF-kB and ERK1/2 pathways in colon cancer cells.
Methods: Human CRC cell lines HT29 and HCT116 were incubated under hypoxic (1% oxygen) and normoxic (21% oxygen) conditions. Western blot analysis and real-time PCR were used to evaluate the expression of EMT markers in the presence of KRGE. Furthermore, we performed scratched wound healing, transwell migration, and invasion assays to monitor whether KRGE affects migratory and invasive abilities of CRC cells under hypoxic conditions.
Results: KRGE-treated HT29 and HCT116 cells displayed attenuated vascular endothelial growth factor (VEGF) mRNA levels and hypoxia-inducible factor-1a (HIF-1a) protein expression under hypoxic conditions. KRGE repressed Snail, Slug, and Twist mRNA expression and integrin aVb6 protein levels. Furthermore, hypoxia-repressed E-cadherin was restored in KRGE-treated cells; KRGE blocked the invasion and migration of colon cancer cells by repressing NF-kB and ERK1/2 pathways in hypoxia.
Conclusions: KRGE inhibits hypoxia-induced EMT by repressing NF-kB and ERK1/2 pathways in colon cancer cells.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] - 정규원 / 2013 / Cancer Statistics in Korea: Incidence, Mortality, Survival and Prevalence in 2010 / Cancer Research and Treatment 45 (1) : 1 ~ 14
[단행본] - Howlader N / 2014 / SEER cancer statistics review / National Cancer Institute : 1975 ~ 2011
[학술지(정기간행물)] - Tang CM / 2013 / Hypoxia-inducible factor-1 as a therapeutic target in cancer / J Gastroenterol Hepatol 28 : 401 ~ 405
[학술지(정기간행물)] - Keith B / 2007 / Hypoxia-inducible factors, stem cells, and cancer / Cell 129 : 465 ~ 472
[학술지(정기간행물)] - Bao B / 2012 / The biological kinship of hypoxia with CSC and EMT and their relationship with deregulated expression of miRNAs and tumor aggressiveness / Biochim Biophys Acta 1826 : 272 ~ 296
[단행본] - Howlader N / 2014 / SEER cancer statistics review / National Cancer Institute : 1975 ~ 2011
[학술지(정기간행물)] - Tang CM / 2013 / Hypoxia-inducible factor-1 as a therapeutic target in cancer / J Gastroenterol Hepatol 28 : 401 ~ 405
[학술지(정기간행물)] - Keith B / 2007 / Hypoxia-inducible factors, stem cells, and cancer / Cell 129 : 465 ~ 472
[학술지(정기간행물)] - Bao B / 2012 / The biological kinship of hypoxia with CSC and EMT and their relationship with deregulated expression of miRNAs and tumor aggressiveness / Biochim Biophys Acta 1826 : 272 ~ 296
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Prophylactic role of Korean Red Ginseng in astrocytic mitochondrial biogenesis through HIF-1a
Journal of Ginseng Research
2022 .05
-
Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice
Journal of Ginseng Research
2023 .09
-
Korean Red Ginseng-induced astrocytic HIF-1α: A key regulator of neuroglobin derived from neural stem cell differentiation in physiologic retina and brain
Journal of Ginseng Research
2025 .03
-
Multitarget effects of Korean Red Ginseng in animal model of Parkinson`s disease : antiapoptosis, antioxidant, antiinflammation, and maintenance of blood-brain barrier integrity
Journal of Ginseng Research
2018 .06
-
Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway
Journal of Ginseng Research
2021 .03
-
Korean Red Ginseng alleviates dehydroepiandrosterone-induced polycystic ovarian syndrome in rats via its antiinflammatory and antioxidant activities
Journal of Ginseng Research
2020 .11
-
Korean Red Ginseng extract and ginsenoside Rg3 have anti-pruritic effects on chloroquine-induced itch by inhibition of MrgprA3/TRPA1-mediated pathway
Journal of Ginseng Research
2018 .10
-
Bone remodeling effects of Korean Red Ginseng extracts for dental implant applications
Journal of Ginseng Research
2020 .11
-
Immunological benefits by ginseng through reciprocal regulation of Th17 and Treg cells during cyclosporine-induced immunosuppression
Journal of Ginseng Research
2016 .01
-
Ovarian cysts disappear after 14-day oral regimen of Korean red ginseng extract in letrozole-induced polycystic ovarian syndrome
Obstetrics & Gynecology Science
2021 .01
-
Hypoxia Induces Epithelial-Mesenchymal Transition in Follicular Thyroid Cancer: Involvement of Regulation of Twist by Hypoxia Inducible Factor-1α
Yonsei Medical Journal
2015 .01
-
Rg3-enriched Korean Red Ginseng extract inhibits blood-brain barrier disruption in an animal model of multiple sclerosis by modulating expression of NADPH oxidase 2 and 4
Journal of Ginseng Research
2021 .05
-
Respiratory protective effects of Korean Red Ginseng in a mouse model of particulate matter 4-induced airway inflammation
Journal of Ginseng Research
2023 .01
-
Enhanced Rg3 negatively regulates Th1 cell responses
Journal of Ginseng Research
2019 .01
-
Effect of Korean Red Ginseng extract on colorectal lung metastasis through inhibiting the epitheliale-mesenchymal transition via transforming growth factor-β1/Smad-signaling-mediated Snail/E-cadherin expression
Journal of Ginseng Research
2019 .01
-
Early Growth Response Protein-1 Involves in Transforming Growth factor-β1 Induced Epithelial-Mesenchymal Transition and Inhibits Migration of Non-Small-Cell Lung Cancer Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Korean Red Ginseng mitigates spinal demyelination in a model of acute multiple sclerosis by downregulating p38 mitogen-activated protein kinase and nuclear factor-kB signaling pathways
Journal of Ginseng Research
2018 .10
-
miR-485 Inhibits Epithelial-Mesenchymal Transition, Migration and Invasion of Pancreatic Cancer Cells by Directly Repressing PAK1
대한외과학회 학술대회 초록집
2018 .11
-
Attitudes Towards Colorectal Cancer (CRC) and CRC Screening Tests among Elderly Malay Patients
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Genipin inhibits hypoxia-induced accumulation of HIF-1α and VEGF expression in human cervical carcinoma HeLa cells
고신대학교 의과대학 학술지
2019 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
HaCaT 인간 각질세포에서 산화적 스트레스에 대한 머루근 수용성 추출물의 세포 보호 효과
최은옥
,
권다혜
,
황보현
외 8명
한국식품영양과학회지
2018
.01
유방암세포에서 괴화 에탄올 추출물의 NF-kB 의존적인 MMP-9 발현의 조절 규명을 위한 연구
김정미
,
이영래
,
황진기
외 7명
동의생리병리학회지
2014
.02
Effects of Aucubin Isolated from Eucommia ulmoides on UVB-induced Oxidative Stress in Human Keratinocytes HaCaT
Jin Nyoung Ho
,
Hong Yon Cho
,
Eun Jeong Lim
외 1명
Food Science and Biotechnology
2009
.04
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-524-003161155