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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.45 No.3
- 발행연도
- 2018.9
- 수록면
- 385 - 400 (16page)
이용수
초록· 키워드
오류제보하기
Marker-assisted backcrossing (MABC) is useful for selecting offspring with a highly recovered genetic background for a recurrent parent at early generation unlike rice and other field crops. Molecular marker sets applicable to practical MABC are scarce in vegetable crops including tomatoes. In this study, we used the National Center for Biotechnology Information- short read archive (NCBI-SRA) database that provided the whole genome sequences of 234 tomato accessions and selected 27,680 tag-single nucleotide polymorphisms (tag-SNPs) that can identify haplotypes in the tomato genome. From this SNP dataset, a total of 143 tag-SNPs that have a high polymorphism information content (PIC) value (> 0.3) and are physically evenly distributed on each chromosome were selected as a MABC marker set. This marker set was tested for its polymorphism in each pairwise cross combination constructed with 124 of the 234 tomato accessions, and a relatively high number of SNP markers polymorphic for the cross combination was observed. The reliability of the MABC SNP set was assessed by converting 18 SNPs into Luna probe-based high-resolution melting (HRM) markers and genotyping nine tomato accessions. The results show that the SNP information and HRM marker genotype matched in 98.6% of the experiment data points, indicating that our sequence analysis pipeline for SNP mining worked successfully. The tag-SNP set for the MABC developed in this study can be useful for not only a practical backcrossing program but also for cultivar identification and F1 seed purity test in tomatoes.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)] - 배중환 / 2010 / 토마토 품종 구분을 위한 SNP 분자표지 개발 / 원예과학기술지 28 (4) : 627 ~ 637
[학술지(정기간행물)] - Birrer M / 2014 / A DNA marker assay based on high-resolution melting curve analysis for distinguishing species of the Festuca-Lolium complex / Molecular Breeding 34 : 421 ~ 429
[학술지(정기간행물)] - Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphisms / American Society of Human Genetics 32 : 314 ~ 331
[학술지(정기간행물)] - Causse M / 2013 / Whole genome resequencing in tomato reveals variation associated with introgression and breeding events / BMC Genomics 14 : 791 ~
[학술지(정기간행물)] - Collard BC / 2008 / Marker-assisted selection: An approach for precision plant breeding in the twenty-first century / Philosophical Transactions of the Royal Society B 363 : 557 ~ 572
[학술지(정기간행물)] - Birrer M / 2014 / A DNA marker assay based on high-resolution melting curve analysis for distinguishing species of the Festuca-Lolium complex / Molecular Breeding 34 : 421 ~ 429
[학술지(정기간행물)] - Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphisms / American Society of Human Genetics 32 : 314 ~ 331
[학술지(정기간행물)] - Causse M / 2013 / Whole genome resequencing in tomato reveals variation associated with introgression and breeding events / BMC Genomics 14 : 791 ~
[학술지(정기간행물)] - Collard BC / 2008 / Marker-assisted selection: An approach for precision plant breeding in the twenty-first century / Philosophical Transactions of the Royal Society B 363 : 557 ~ 572
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UCI(KEPA) : I410-ECN-0101-2018-520-003591271