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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 165 - 171 (7page)
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초록· 키워드
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Marker-assisted backcrossing (MABC) is useful for selecting an offspring with a highly recovered genetic background for a recurrent parent at early generation to various crops. Moreover, marker-assisted backcrossing (MABC) along with marker-assisted selection (MAS) contributes immensely to overcome the main limitation of the conventional breeding and it accelerates recurrent parent genome (RPG) recovery. In this study, we were employed to incorporate rin gene(s) from the donor parent T13-1084, into the genetic background of HK13-1151, a popular highyielding tomato elite inbred line that is a pink color fruit, in order to develop a rin HK13-1084 improved line. The recurrent parent genome recovery was analyzed in early generations of backcrossing using SNP markers obtained from genotyping-by-sequencing analysis. From the BC1F1 and BC2F1 plants, 3,086 and 4868 polymorphic SNP markers were obtained via GBS analysis, respectively. These markers were present in all twelve chromosomes. The background analysis revealed that the extent of RPG recovery ranged from 56.7% to 84.5% and from 87.8% to 97.8% in BC1F1 and BC2F1 generations, respectively. In this study, No 5-1 with 97.8% RPG recovery rate among BC2F1 plants was similar to HK13-1151 strain in the fruit shape. Therefore, the selected plants were fixed in BC2F2 generation through selfing. MAS allowed identification of the plants that are more similar to the recurrent parent for the loci evaluated in the backcross generations. MABC can greatly reduce breeding time as compared to the conventional backcross breeding. For instance, MABC approach greatly shortened breeding time in tomato.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
안송지 / 2010 / 멜론 및 참외 순도 검정을 위한 SNP 마커 개발 및 F1 종자 순도 검정 / 한국육종학회지 42(4):397~406
Chagné D / 2012 / Genome-wide SNP detection, validation, and development of an 8K SNP array for apple. / PLoS One 7(2):e31745
Collard BC / 2008 / Mackill DJ(2008)Marker-assisted selection : An approach for precision plant breeding in the twenty-first century / Philosophical Transactions of the Royal Society B 363:557~555
Consortium TG / 2012 / The tomato genome sequence provides insights into fleshy fruit evolution / Nature 485(7400):635~641
Cuesta-Marcos A / 2010 / Genome-wide SNPs and re-sequencing of growth habit and inflorescence genes in barley: implications for association mapping in germplasm arrays varying in size and structure / BMC Genomics 11(1):707
Chagné D / 2012 / Genome-wide SNP detection, validation, and development of an 8K SNP array for apple. / PLoS One 7(2):e31745
Collard BC / 2008 / Mackill DJ(2008)Marker-assisted selection : An approach for precision plant breeding in the twenty-first century / Philosophical Transactions of the Royal Society B 363:557~555
Consortium TG / 2012 / The tomato genome sequence provides insights into fleshy fruit evolution / Nature 485(7400):635~641
Cuesta-Marcos A / 2010 / Genome-wide SNPs and re-sequencing of growth habit and inflorescence genes in barley: implications for association mapping in germplasm arrays varying in size and structure / BMC Genomics 11(1):707
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