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논문 기본 정보

자료유형
학술저널
저자정보
Jae Sik Yu (Sungkyunkwan University) Hyun-Soo Roh (Sungkyunkwan University School of Medicine) Kwan-Hyuck Baek (Sungkyunkwan University School of Medicine) Seul Lee (Sungkyunkwan University School of Medicine) Sil Kim (Sungkyunkwan University) Hae Min So (Sungkyunkwan University) Eunjung Moon (Charmzone Co) Changhyun Pang (Sungkyunkwan University) Tae Su Jang (Seoul National University) Ki Hyun Kim (Sungkyunkwan University)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.42 No.4
발행연도
2018.10
수록면
562 - 570 (9page)

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Background: Lung cancer is the leading cause of cancer-related death worldwide. In this study, we used a bioactivity-guided isolation technique to identify constituents of Korean Red Ginseng (KRG) with antiproliferative activity against human lung adenocarcinoma cells.
Methods: Bioactivity-guided fractionation and preparative/semipreparative HPLC purification were used with LC/MS analysis to separate the bioactive constituents. Cell viability and apoptosis in human lung cancer cell lines (A549, H1264, H1299, and Calu-6) after treatment with KRG extract fractions and constituents thereof were assessed using the water-soluble tetrazolium salt (WST-1) assay and terminal deoxyribonucleotidyl transferaseemediated dUTP nick end labeling (TUNEL) staining, respectively. Caspase activation was assessed by detecting its surrogate marker, cleaved poly adenosine diphosphate (ADP-ribose) polymerase, using an immunoblot assay. The expression and subcellular localization of apoptosis-inducing factor were assessed using immunoblotting and immunofluorescence, respectively.
Results and conclusion: Bioactivity-guided fractionation of the KRG extract revealed that its ethyl acetatee soluble fraction exerts significant cytotoxic activity against all human lung cancer cell lines tested by inducing apoptosis. Chemical investigation of the ethyl acetatesoluble fraction led to the isolation of six ginsenosides, including ginsenoside Rb1 (1), ginsenoside Rb2 (2), ginsenoside Rc (3), ginsenoside Rd (4), ginsenoside Rg1 (5), and ginsenoside Rg3 (6). Among the isolated ginsenosides, ginsenoside Rg3 exhibited the most cytotoxic activityagainst all human lung cancer cell lines examined, with IC<SUB>50</SUB> values ranging from161.1 mMto 264.6 mM. The cytotoxicity of ginsenoside Rg3 was found to be mediated by induction of apoptosis in a caspaseindependent manner. These findings provide experimental evidence for a novel biological activity of ginsenoside Rg3 against human lung cancer cells.

목차

abstract
1. Introduction
2. Materials and methods
3. Results and discussion
References

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