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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 562 - 570 (9page)
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초록· 키워드
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Background: Lung cancer is the leading cause of cancer-related death worldwide. In this study, we used a bioactivity-guided isolation technique to identify constituents of Korean Red Ginseng (KRG) with antiproliferative activity against human lung adenocarcinoma cells.
Methods: Bioactivity-guided fractionation and preparative/semipreparative HPLC purification were used with LC/MS analysis to separate the bioactive constituents. Cell viability and apoptosis in human lung cancer cell lines (A549, H1264, H1299, and Calu-6) after treatment with KRG extract fractions and constituents thereof were assessed using the water-soluble tetrazolium salt (WST-1) assay and terminal deoxyribonucleotidyl transferaseemediated dUTP nick end labeling (TUNEL) staining, respectively. Caspase activation was assessed by detecting its surrogate marker, cleaved poly adenosine diphosphate (ADP-ribose) polymerase, using an immunoblot assay. The expression and subcellular localization of apoptosis-inducing factor were assessed using immunoblotting and immunofluorescence, respectively.
Results and conclusion: Bioactivity-guided fractionation of the KRG extract revealed that its ethyl acetatee soluble fraction exerts significant cytotoxic activity against all human lung cancer cell lines tested by inducing apoptosis. Chemical investigation of the ethyl acetatesoluble fraction led to the isolation of six ginsenosides, including ginsenoside Rb1 (1), ginsenoside Rb2 (2), ginsenoside Rc (3), ginsenoside Rd (4), ginsenoside Rg1 (5), and ginsenoside Rg3 (6). Among the isolated ginsenosides, ginsenoside Rg3 exhibited the most cytotoxic activityagainst all human lung cancer cell lines examined, with IC<SUB>50</SUB> values ranging from161.1 mMto 264.6 mM. The cytotoxicity of ginsenoside Rg3 was found to be mediated by induction of apoptosis in a caspaseindependent manner. These findings provide experimental evidence for a novel biological activity of ginsenoside Rg3 against human lung cancer cells.
Methods: Bioactivity-guided fractionation and preparative/semipreparative HPLC purification were used with LC/MS analysis to separate the bioactive constituents. Cell viability and apoptosis in human lung cancer cell lines (A549, H1264, H1299, and Calu-6) after treatment with KRG extract fractions and constituents thereof were assessed using the water-soluble tetrazolium salt (WST-1) assay and terminal deoxyribonucleotidyl transferaseemediated dUTP nick end labeling (TUNEL) staining, respectively. Caspase activation was assessed by detecting its surrogate marker, cleaved poly adenosine diphosphate (ADP-ribose) polymerase, using an immunoblot assay. The expression and subcellular localization of apoptosis-inducing factor were assessed using immunoblotting and immunofluorescence, respectively.
Results and conclusion: Bioactivity-guided fractionation of the KRG extract revealed that its ethyl acetatee soluble fraction exerts significant cytotoxic activity against all human lung cancer cell lines tested by inducing apoptosis. Chemical investigation of the ethyl acetatesoluble fraction led to the isolation of six ginsenosides, including ginsenoside Rb1 (1), ginsenoside Rb2 (2), ginsenoside Rc (3), ginsenoside Rd (4), ginsenoside Rg1 (5), and ginsenoside Rg3 (6). Among the isolated ginsenosides, ginsenoside Rg3 exhibited the most cytotoxic activityagainst all human lung cancer cell lines examined, with IC<SUB>50</SUB> values ranging from161.1 mMto 264.6 mM. The cytotoxicity of ginsenoside Rg3 was found to be mediated by induction of apoptosis in a caspaseindependent manner. These findings provide experimental evidence for a novel biological activity of ginsenoside Rg3 against human lung cancer cells.
목차
abstract
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (54)
참고문헌 신청
[학술지(정기간행물)] - Jemal A / 2010 / Cancer statistics / CA Cancer J Clin 60 : 277 ~ 300
[학술지(정기간행물)] - 백인호 / 2013 / The world ginseng market and the ginseng (Korea) / Journal of Ginseng Research 37 (1) : 1 ~ 7
[학술지(정기간행물)] - Kim P / 2013 / Effects of Korean red ginseng extracts on neural tube defects and impairment of social interaction induced by prenatal exposure to valproic acid / Food Chem Toxicol 51 : 288 ~ 296
[학술지(정기간행물)] - Park HM / 2012 / Korean red ginseng and its primary ginsenosides inhibit ethanolinduced oxidative injury by suppression of the MAPK pathway in TIB-73 cells / J Ethnopharmacol 141 : 1071 ~ 1076
[학술지(정기간행물)] - Paul S / 2012 / Inhibition of inflammations and macrophage activation by ginsenoside-Re isolated from Korean ginseng (Panax ginseng C.A. Meyer) / Food Chem Toxicol 50 : 1354 ~ 1361
[학술지(정기간행물)] - 백인호 / 2013 / The world ginseng market and the ginseng (Korea) / Journal of Ginseng Research 37 (1) : 1 ~ 7
[학술지(정기간행물)] - Kim P / 2013 / Effects of Korean red ginseng extracts on neural tube defects and impairment of social interaction induced by prenatal exposure to valproic acid / Food Chem Toxicol 51 : 288 ~ 296
[학술지(정기간행물)] - Park HM / 2012 / Korean red ginseng and its primary ginsenosides inhibit ethanolinduced oxidative injury by suppression of the MAPK pathway in TIB-73 cells / J Ethnopharmacol 141 : 1071 ~ 1076
[학술지(정기간행물)] - Paul S / 2012 / Inhibition of inflammations and macrophage activation by ginsenoside-Re isolated from Korean ginseng (Panax ginseng C.A. Meyer) / Food Chem Toxicol 50 : 1354 ~ 1361
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