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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 435 - 438 (4page)
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초록· 키워드
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Acanthamoeba culbertsoni is the causative agent of granulomatous amoebic encephalitis (GAE), a condition that predominantly occurs in immunocompromised individuals and which is typically fatal. A mannose-binding protein (MBP) among lectins was shown to have strong A. castellanii pathogenic potential when correlated with major virulence proteins. In this study, protective effects were analyzed using the monoclonal antibody to A. culbertsoni MBP by quantification and were also compared with other free-living amoebae. For the amoebial cytotoxicity to the target cell, amoeba trophozoites were incubated with Chinese hamster ovary (CHO) cells. For the protective effects of antibodies, amoebae were preincubated with them for 4 h and then added to the target cells. After 24 h, the supernatants were collected and examined for host cell cytotoxicity by measuring lactate dehydrogenase (LDH) release. The cytotoxicity of A. culbertsoni to the CHO cells showed about 87.4%. When the monoclonal antibody was pre-incubated with A. culbertsoni, the amoebial cytotoxicity was remarkably decreased as shown at LDH release (1.858 absorbance), which was represented with about 49.9%. Taken together, it suggested that the monoclonal antibody against MBP be important to inhibit the cytotoxicity of A. culbertsoni trophozoites to the target cell. The antibody will be applied into an in vivo functional analysis, which would help to develop therapeutics.
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참고문헌 (12)
참고문헌 신청
[학술지(정기간행물)] - Garate M / 2004 / Cloning and characterization of a novel mannose-binding protein of Acanthamoeba / J Clin Chem 279 : 29849 ~ 29856
[학술지(정기간행물)] - Kang AY / 2018 / Production of a monoclonal antibody against a mannosebinding protein of Acanthamoeba culbertsoni and its localization / Exp Parasitol 192 : 19 ~ 24
[학술지(정기간행물)] - Kang SY / 2005 / Role of the Nfa1 protein in pathogenic Naegleria fowleri cocultured with CHO target cells / Clin Diagn Lab Immunol 12 : 873 ~ 876
[학술지(정기간행물)] - Khan NA / 2006 / Acanthamoeba: biology and increasing importance in human health / FEMS Microbiol Rev 30 : 564 ~ 595
[학술지(정기간행물)] - Kim JH / 2012 / Functional roles of mannose-binding protein in the adhesion, cytotoxicity and phagocytosis of Acanthamoeba castellanii / Exp Parasitol 132 : 287 ~ 292
[학술지(정기간행물)] - Kang AY / 2018 / Production of a monoclonal antibody against a mannosebinding protein of Acanthamoeba culbertsoni and its localization / Exp Parasitol 192 : 19 ~ 24
[학술지(정기간행물)] - Kang SY / 2005 / Role of the Nfa1 protein in pathogenic Naegleria fowleri cocultured with CHO target cells / Clin Diagn Lab Immunol 12 : 873 ~ 876
[학술지(정기간행물)] - Khan NA / 2006 / Acanthamoeba: biology and increasing importance in human health / FEMS Microbiol Rev 30 : 564 ~ 595
[학술지(정기간행물)] - Kim JH / 2012 / Functional roles of mannose-binding protein in the adhesion, cytotoxicity and phagocytosis of Acanthamoeba castellanii / Exp Parasitol 132 : 287 ~ 292
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UCI(KEPA) : I410-ECN-0101-2019-510-000302696