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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 101 - 108 (8page)
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The Agrobacterium-mediated transformation has been successfully used method to introduce foreign genes into some monocotyledonous as well as a large number of dicotyledonous plants genome. We developed transgenic Chinese cabbage plants with insect-resistance gene, modified CryIAc, by Agrobacterium- transformation and confirmed transgene copy number by Southern blot analysis. We confirmed that twenty-nine out of 46 transgenic Chinese cabbage plants have single copy of CryIAc. To obtain the sequences information on the transferred DNA (T-DNA) integration into plant genome, we analyzed left border (LB) flanking sequences by genome walking (GW) PCR method. Out of 46 transgenic Chinese cabbage plants examined, 37 carried the vector backbone sequences. This result indicates that the transfer of the vector backbone from the binary vectors resulted mainly from inefficient termination of LB site. Analysis of T-DNA LB flanking region of 9 transgenic Chinese cabbage plants without vector backbone revealed that all LB ends were not conserved and nucleotides up to 36bp from the LB cleavage site were deleted.
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참고문헌 (24)
참고문헌 신청
Abdal-Aziz S. A. / 2006 / Evidence of Frequent Integration of Non-T-DNA Vector Backbone Sequences in Transgenic Strawberry / Plant. J Biosci Bioeng 101 : 508 ~ 510
Alonso J. M. / 2003 / Genome-wide insertional mutagenesis of Arabidopsis thaliana / Science 301 : 653 ~ 657
Cho H. S. / 2001 / Control of lepidopteran insect pests in transgenic Chinese cabbage transformed with synthetic Bacillus thuringiensis cry1C gene / Plant Cell Rep 20 : 1 ~ 7
De Buck S. / 2000 / T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation / Mol Bre
Gamble J. / 2002 / The New Zealand public’s attitudes regarding genetically modified fo
Alonso J. M. / 2003 / Genome-wide insertional mutagenesis of Arabidopsis thaliana / Science 301 : 653 ~ 657
Cho H. S. / 2001 / Control of lepidopteran insect pests in transgenic Chinese cabbage transformed with synthetic Bacillus thuringiensis cry1C gene / Plant Cell Rep 20 : 1 ~ 7
De Buck S. / 2000 / T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation / Mol Bre
Gamble J. / 2002 / The New Zealand public’s attitudes regarding genetically modified fo
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