지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 186 - 195 (10page)
이용수
초록· 키워드
오류제보하기
Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive α subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation.
Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis.
Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3- acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Bartel B / 1997 / Auxin biosynthesis / Annu. Rev. Plant Physiol. Plant Mol. Biol 48 : 51 ~ 66
Bender J / 1998 / A myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis / Proceedings of the National Academy of Sciences, USA 95 : 5655 ~ 5660
Bohlmann J / 1995 / Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: modes of expression and properties of native and recombinant enzymes / Plant
Hattori A / 1995 / Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates / Biochemistry and Molec
Huber W / 2002 / Variance stabilization applied to microarray data calibration and to the quantification of differential expression / Bioinformatics 18 (1) : S96 ~ S104
Bender J / 1998 / A myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis / Proceedings of the National Academy of Sciences, USA 95 : 5655 ~ 5660
Bohlmann J / 1995 / Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: modes of expression and properties of native and recombinant enzymes / Plant
Hattori A / 1995 / Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates / Biochemistry and Molec
Huber W / 2002 / Variance stabilization applied to microarray data calibration and to the quantification of differential expression / Bioinformatics 18 (1) : S96 ~ S104
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Tryptophan Metabolites and the Gut Microbiota Associated with Human Metabolic Health in Korean Adults
한국식품영양과학회 학술대회발표집
2022 .10
-
산란계 평사 사육시 산란말기 L-tryptophan 급여 수준이 생산성, 깃털손상도 및 스트레스 수준에 미치는 영향
한국가금학회 정기총회 및 학술발표회
2021 .11
-
Optimization of Indole-3-acetic Acid (IAA) Production by Bacillus megaterium BM5
한국토양비료학회지
2016 .10
-
Effects of arginine, tryptophan, and threonine on chicken intestinal epithelial cell induced with oxidative stress
한국가금학회 정기총회 및 학술발표회
2024 .11
-
레스베라트롤 합성 GM 벼 검정을 위한 계통특이 마커 개발
한국육종학회지
2016 .01
-
“Dietary supplementation of L-tryptophan” increases muscle development, adipose tissue catabolism and fatty acid transportation in the muscles of Hanwoo steers
한국축산학회지
2020 .01
-
형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석
한국육종학회지
2015 .01
-
해충저항성 유전자변형 벼(Agb0101) 유전자 이동성 평가
한국육종학회지
2017 .01
-
CRISPR/Cas9‑targeted mutagenesis of F3′H, DFR and LDOX, genes related to anthocyanin biosynthesis in black rice (Oryza sativa L.)
Plant Biotechnology Reports
2019 .01
-
Mitigative Role of Auxin Against Oxidative Stress in Rice (Oryza Sativa L.) Seedlings
한국토양비료학회 학술발표회 초록집
2022 .10
-
Mitigative Role of Auxin Against Oxidative Stress in Rice (Oryza Sativa L.) Seedlings
한국토양비료학회 학술발표회 초록집
2022 .10
-
Gene Flow from Transgenic Rice to Conventional Rice in China
Plant Breeding and Biotechnology
2021 .12
-
식물에서 shikimate 및 방향족 아미노산 생합성 경로와 이의 대사공학적 응용
Journal of Plant Biotechnology
2015 .01
-
Transcriptome profiling of the coffee (C. arabica L.) seedlings under salt stress condition
Journal of Plant Biotechnology
2018 .01
-
Comparative nutritional analysis for protopanaxadiol-enhanced genetically modified rice and its non-transgenic counterpart
Korean Journal of Agricultural Science
2024 .06
-
Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines
Plant Biotechnology Reports
2020 .01
-
Development of Resistant Gene-Pyramided Japonica Rice for Multiple Biotic Stresses Using Molecular Marker-Assisted Selection
Plant Breeding and Biotechnology
2015 .01
-
Comparing the profiles of GM rice (insect tolerance) with non-GM and commercial rice varieties
한국식품영양과학회 학술대회발표집
2016 .10
-
β-카로틴 강화벼의 분자생물학적 특성과 안전성 평가
한국육종학회지
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0