지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 461 - 468 (8page)
이용수
초록· 키워드
오류제보하기
One of the important phytohormones produced by plant growth promoting bacteria is the auxin; indole-3-acetic acid (IAA), with L-tryptophan as the precursor. In this study, we focused on the investigation of optimal conditions for the production of IAA by Bacillus megaterium BM5. We investigated culturing conditions, such as incubation temperature, pH of the culture medium and incubation period, with varying media components such as inoculation volume, tryptophan concentration and carbon and nitrogen source. Besides, optimization study intended for high IAA production was carried out with fermentation parameters such as rpm and aeration. The initial yield of 42 μg IAA ml<SUP>-1</SUP> after 24 hr increased to 85 μg ml<SUP>-1</SUP> when 5% (v/v) of L-tryptophan was used in the culture broth. The maximum yield of 320 μg IAA ml<SUP>-1</SUP> was observed in trypticase soy broth (TSB) supplemented with starch and soybean meal as C and N sources with a C/N ratio of 3:1 (v/v) at 30°C, pH 8.0 for 48 hrs with 1.0 vvm and 250 rpm in 5 L working volume using 10 L scale fermenter. The bacterial auxin extracted from the culture broth was confirmed by thin layer chromatography and high-performance liquid chromatography and effect on plant growth was confirmed by root elongation test.
목차
Introduction
Materials and Methods
Results and Discussion
Conclusions
References
참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)] - Barea, J.M. / 1976 / Production of plant growth regulators by rhizosphere phosphate solubilizing bacteria / J. Appl. Bacteriol 40 : 129 ~ 134
[단행본] - Davies, P.J. / 1995 / Plant Hormones Physiology, Biochemistry and Molecular Biology / Kluwer Academic Publishers : 1 ~ 12
[학술지(정기간행물)] - Denvender, K.J. / 1984 / Characterization of a substance produced by Azospirillum which causes branching of wheat root hairs / Can. J. Microbiol 31 : 206 ~ 210
[단행본] - Frankenberger, W.T. / 1995 / Phytohormones in soil: Microbial production and function / Marcel Dekker Inc : 503 ~
[학술지(정기간행물)] - Glick, B.R. / 1995 / The enhancement of plant growth by free-living bacteria / Can. J. Microbiol 41 : 680 ~ 685
[단행본] - Davies, P.J. / 1995 / Plant Hormones Physiology, Biochemistry and Molecular Biology / Kluwer Academic Publishers : 1 ~ 12
[학술지(정기간행물)] - Denvender, K.J. / 1984 / Characterization of a substance produced by Azospirillum which causes branching of wheat root hairs / Can. J. Microbiol 31 : 206 ~ 210
[단행본] - Frankenberger, W.T. / 1995 / Phytohormones in soil: Microbial production and function / Marcel Dekker Inc : 503 ~
[학술지(정기간행물)] - Glick, B.R. / 1995 / The enhancement of plant growth by free-living bacteria / Can. J. Microbiol 41 : 680 ~ 685
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UCI(KEPA) : I410-ECN-0101-2017-523-001637953