지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 341 - 348 (8page)
이용수
초록· 키워드
오류제보하기
Purpose: Tumor necrosis factor (TNF)-related apoptosis
inducing ligand (TRAIL)/APO-2L is a member of the
TNF family that can kill a wide variety of tumor cells, but
not normal cells. This study was designed to investigate
the down stream target proteins in TRAIL-mediated apoptosis
of human liver, Chang cells.
Materials and Methods: The expressions of DR4/DR5
in hepatoma cells, including Chang, HepG2 and Hep3B
cells, were determined by RT-PCR. Cell viability was measured
by MTT assay and apoptosis was assessed by DNA
fragmentation assay. The catalytic activity of caspasefamily
proteases, including caspase-3 and -9, was tested
by using fluorogenic biosubstrates. Expression of apoptotic
mediators, including procaspase-3 and PARP proteins,
was measured by Western blotting. The expression
profile of proteins in Chang cells by using two-dimensional
(2-D) gel electrophoresis and MALDI-TOF.
Results: The results demonstrated that TRAIL (100 ng/
ml) induced the apoptotic death of Chang cells, as characterized
by the ladder-pattern fragmentation of genomic
DNA. TRAIL increased the enzymatic activity of caspase-
3, corresponding to the time of appearance of cleaved
PARP and caspase-9. In 2-D gel electrophoresis and MALDITOF
analysis, the comparison of control versus apoptotic
cells in the protein expressions revealed that signal
intensity of 7 spots were decreased, whereas 6 spots were
increased among 300 spots. These spots were resolved and
identified as a protein information by MALDI-TOF.
Conclusion: We suggested that TRAIL induces the apoptotic
death of Chang cells via proteome alterations inducing
caspase cascade. (Cancer Res Treat. 2003;35:341-348)
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effect of Arsenic Trioxide in TRAIL (Tumor Necrosis Factor-related Apoptosis Inducing Ligand)-Mediated Apoptosis in Multiple Myeloma Cell Lines
Cancer Research and Treatment
2003 .01
-
Effect of Snake Venom Toxin on Inhibition of Colorectal Cancer HT29 Cells Growth via Death Receptors Mediated Apoptosis
대한침구의학회지
2014 .01
-
Over Expression of BCL2 and Low Expression of Caspase 8 Related to TRAIL Resistance in Brain Cancer Stem Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
The Combination of TRAIL Treatment and Cancer Cell Selective Expression of TRAIL-Death Receptor DR4 Induces Cell Death in TRAIL-Resistant Cancer Cells
Yonsei Medical Journal
2006 .01
-
Butein Sensitizes Human Leukemia Cells to Apoptosis Induced by Tumor Necrosis Factor-related Apoptosis Inducing Ligand (TRAIL)
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2008 .01
-
Hydrogen Peroxide Upregulates TNF-Related Apoptosis-Inducing Ligand (TRAIL) Expression in Human Astroglial Cells, and Augments Apoptosis of T Cells
Yonsei Medical Journal
2006 .01
-
Down-Regulation of Survivin by Nemadipine-A Sensitizes Cancer Cells to TRAIL-Induced Apoptosis
Biomolecules & Therapeutics(구 응용약물학회지)
2013 .01
-
Up-regulation of the DR5 Expression by Proteasome Inhibitor MG132 Augments TRAIL-Induced Apoptosis in Soft Tissue Sarcoma Cell Lines
Cancer Research and Treatment
2011 .01
-
Expression of Bcl-2 and Caspase-3 Proteins Related to Apoptosis in Human Leukemia K-562 Cells
대한의생명과학회지
2005 .09
-
Dealing Naturally with Stumbling Blocks on Highways and Byways of TRAIL Induced Signaling
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
Inhibitory Effect of Snake Venom Toxin on Colorectal Cancer HCT116 Cells Growth through Induction of Intrinsic or Extrinsic Apoptosis
대한침구의학회지
2013 .01
-
Apigenin Sensitizes Huh-7 Human Hepatocellular Carcinoma Cells to TRAIL-induced Apoptosis
Biomolecules & Therapeutics(구 응용약물학회지)
2012 .01
-
인체 간암세포에서 Apigenin에 의한 TRAIL 유도 Apoptosis의 증진 효과
약학회지
2011 .01
-
TRAIL Based Therapy: Overview of Mesenchymal Stem Cell Based Delivery and miRNA Controlled Expression of TRAIL
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
TRAIL in Combination with Subtoxic 5-FU Effectively Inhibit Cell Proliferation and Induce Apoptosis in Cholangiocarcinoma Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
EJ 인간 방광암 세포에서 bufalin 의 TRAIL 저항성 극복 효과
대한한의학방제학회지
2017 .01
-
Arsenic Trioxide Induces Apoptosis of HL-60 Cells via Activation of Intrinsic Caspase Protease with MitochondrialDysfunction
Cancer Research and Treatment
2002 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0