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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 869 - 879 (11page)
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Purpose We investigated the effects of laminin on the fraction of cells with self-renewing capacity in the estrogen-dependent, tamoxifen-sensitive LM05-E breast cancer cell line. We also determined whether laminin affected the response to tamoxifen.
Materials and Methods The LM05-E breast cancer cell line was used as a model for all experiments. Aldehyde dehydrogenase (ALDH) activity, clonogenic and mammosphere assays were performed to measure the effects of laminin on modulation of the stem cell subpopulation. Pluripotent gene expression was analyzed by reverse transcriptase–polymerase chain reaction. The involvement of the mitogen-activated protein kinase (MAPK)/ERK pathway was determined using specific inhibitors. The effects of laminin on the response to tamoxifen were determined and the involvement of 6 integrin was investigated.
Results We found that pretreatment with laminin leads to a decrease in cells with the ability to form mammospheres that was accompanied by a decrease in ALDH activity. Moreover, exposure of mammospheres to laminin reduced the capacity to form secondary mammospheres and decreased the expression of Sox-2, Nanog, and Oct-4. We previously reported that 4-OHtamoxifen leads to an increase in the expression of these genes in LM05-E cells. Treatment with signaling pathway inhibitors revealed that the MAPK/ERK pathway mediates the effects of laminin. Finally, laminin induced tamoxifen resistance in LM05-E cells through 6 integrin.
Conclusion Our results suggest that the final number of cells with self-renewing capacity in estrogendependent breast tumors may result from the combined effects of endocrine treatment and microenvironmental cues.
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참고문헌 (24)
참고문헌 신청
Bergamaschi A / 2008 / Extracellular matrix signature identifies breast cancer subgroups with different clinical outcome / J Pathol 214 : 357 ~ 367
Bissell MJ / 2001 / Putting tumours in context / Nat Rev Cancer 1 : 46 ~ 54
Cariati M / 2008 / Alpha-6 integrin is necessary for the tumourigenicity of a stem cell-like subpopulation within the MCF7 breast cancer cell line / Int J Cancer 122 : 298 ~
Finak G / 2008 / Stromal gene expression predicts clinical outcome in breast cancer / Nat Med 14 : 518 ~ 527
Ginestier C / 2007 / ALDH1 is
Bissell MJ / 2001 / Putting tumours in context / Nat Rev Cancer 1 : 46 ~ 54
Cariati M / 2008 / Alpha-6 integrin is necessary for the tumourigenicity of a stem cell-like subpopulation within the MCF7 breast cancer cell line / Int J Cancer 122 : 298 ~
Finak G / 2008 / Stromal gene expression predicts clinical outcome in breast cancer / Nat Med 14 : 518 ~ 527
Ginestier C / 2007 / ALDH1 is
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