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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 589 - 593 (5page)
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초록· 키워드
오류제보하기
Background : The standard PCR with sequence-specific primers (SSP) is a widely used method of HLA-B27 typing in clinical practice. The aim of our study was to evaluate 2 Korean HLA-B27 kits with different real-time PCR chemistries.
Methods : To validate the accuracy of real-time PCR kits, we selected 28 HLA-B27-positive samples and 33 HLA-B27-negative samples with a wide range of different HLA-B specificities typed by standard PCR-SSP. The 2 real-time PCR kits used were the AccuPower� HLA-B27 real-time PCR kit
(Bioneer, Korea) with TaqMan probes and the Real-QTM HLA-B*27 detection kit (BioSewoom, Korea) with SYBR Green I dye for melting curve analysis.
Results : All 61 samples typed by PCR-SSP demonstrated a perfect concordance with the 2 realtime PCR assays. It was possible to clearly discriminate between HLA-B27-positive and -negative samples in both real-time assays.
Conclusions : In summary, both real-time PCR assays for HLA-B27 were fast, reliable, well-adapted for routine laboratory testing, and attractive alternatives to the conventional PCR-SSP method.
목차
등록된 정보가 없습니다.
참고문헌 (12)
참고문헌 신청
Bon MA / 2000 / Genotyping of HLA-B27 by real-time PCR without hybridization probes / Clin Chem 46 : 1000 ~ 1002
Dunky A / 1996 / HLA-B27 determination using serological methods. A comparison of enzyme immunoassay and a microlymphocytotoxic test with flow cytometry and a molecular biolo
Faner R / 2004 / HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR / Hum Immunol 65 : 826 ~ 838
Gonzalez S / 1999 / Immunogenetics, HLA-B27 and spondyloarthropathies / Curr Opin Rheumatol 11 : 257 ~ 264
Khan MA / 2007 / The pathogenetic role of HLA-B27 and its subtypes / Autoimmun Rev 6 : 183 ~ 189
Dunky A / 1996 / HLA-B27 determination using serological methods. A comparison of enzyme immunoassay and a microlymphocytotoxic test with flow cytometry and a molecular biolo
Faner R / 2004 / HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR / Hum Immunol 65 : 826 ~ 838
Gonzalez S / 1999 / Immunogenetics, HLA-B27 and spondyloarthropathies / Curr Opin Rheumatol 11 : 257 ~ 264
Khan MA / 2007 / The pathogenetic role of HLA-B27 and its subtypes / Autoimmun Rev 6 : 183 ~ 189
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