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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 124 - 128 (5page)
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초록· 키워드
오류제보하기
Background: Maintaining the quality of cryopreserved cord blood is crucial. In this pilot study, we describe the results of the internal quality control program for a cord blood bank thus far.
Methods: Donated cord blood units unsuitable for transplantation were selected for internal quality control once a month. One unit of cord blood, aliquoted into 21 capillaries, was cryopreserved and thawed annually to analyze the total nucleated cell count, CD34+ cell count, cell viability test, and colony-forming units assay.
Results: No significant differences in the variables (total nucleated cell count, cell viability, CD34+ cell count) were observed between samples cryopreserved for one and two years. Upon comparing the variables before cryopreservation and post thawing with the capillaries of one year of storage, cell viability and CD34+ cell counts decreased significantly. The use of cord blood samples in capillaries, which can be easily stored for a long period, was similar to the methods used for testing segments attached to the cord blood unit.
Conclusions: The results of this study may be useful for determining the period during which the quality of cryopreserved cord blood units used for transplantation is maintained.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Broxmeyer HE / 2003 / High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years / Proc Natl Acad Sci U
Broxmeyer HE / 2011 / Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopr
Fernyhough LJ / 2013 / Relative recovery of haematopoietic stem cell products after cryogenic storage of up to 19 years / Bone Marrow Transplant 48 : 32 ~ 35
Flores AI / 2009 / Consistency of the initial cell acquisition procedure is critical to the standardization of CD34+ cell enumeration by flow cytometry: results of a pairwis
Gluckman E / 1989 / Hematopoietic reconstitution in a patient with Fanconi’s anemia by means of umbilical-cord blood from an HLA-identical sibling / N Engl J Med 321 : 1174
Broxmeyer HE / 2011 / Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopr
Fernyhough LJ / 2013 / Relative recovery of haematopoietic stem cell products after cryogenic storage of up to 19 years / Bone Marrow Transplant 48 : 32 ~ 35
Flores AI / 2009 / Consistency of the initial cell acquisition procedure is critical to the standardization of CD34+ cell enumeration by flow cytometry: results of a pairwis
Gluckman E / 1989 / Hematopoietic reconstitution in a patient with Fanconi’s anemia by means of umbilical-cord blood from an HLA-identical sibling / N Engl J Med 321 : 1174
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