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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 624 - 629 (6page)
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초록· 키워드
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Background: Several molecular assays have been developed to detect the BRAF V600E mutation in fine needle aspirates (FNAs) for the diagnosis of papillary thyroid cancer. Using a multiplex PCR technique, we evaluated the Anyplex BRAF V600E Real-time Detection (Anyplex) assay and compared its efficacy with that of the Seeplex BRAF V600E ACE Detection (Seeplex) method.
Methods: We tested 258 consecutive FNA specimens using the Seeplex and Anyplex assays. Any conflicting results between the two assays were confirmed by using mutant enrichment with 3´-modified oligonucleotide (MEMO) sequencing. The limits of detection (LODs) and reproducibility for each assay were evaluated with serially diluted DNA from a BRAF V600E-positive cell line.
Results: The BRAF V600E mutation was detected in 36.4% (94/258) FNA specimens by either the Seeplex or Anyplex assay. Results for the two assays showed 93.4% (241/258) agreement, with a kappa value of 0.861 (95% confidence interval, 0.798-0.923). Of the eight specimens that were BRAF V600E-positive by the Anyplex assay but not by the Seeplex assay, five were found to be BRAF V600E-positive by MEMO sequencing. The mutation detection rate of the Seeplex and Anyplex assays was 79.0% and 84.0%, respectively, in the FNA specimens diagnosed as malignant (n=81). The LOD as determined by probit analysis was 0.046% (95% confidence interval, 0.019-0.532%).
Conclusions: The Anyplex assay performed better than the Seeplex assay with respect to the detection of the BRAF V600E mutation.
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참고문헌 (20)
참고문헌 신청
Baloch ZW / 2008 / Diagnostic terminology and morphologic criteria for cytologic diagnosis of thyroid lesions: a synopsis of the National Cancer Institute Thyroid Fine-Needl
Dyhdalo K / 2014 / Assessment of cellularity, genomic DNA yields, and technical platforms for BRAF mutational testing in thyroid fine-needle aspirate samples / Cancer Cytopa
Forbes SA / 2008 / The Catalogue of Somatic Mutations in Cancer (COSMIC) / Curr Protoc Hum Genet
Hertzman Johansson C / 2014 / BRAF inhibitors in cancer therapy / Pharmacol Ther 142 : 176 ~ 182
Jemal A / 2011 / Global cancer statistics / CA Cancer J Clin 61 : 69 ~ 90
Dyhdalo K / 2014 / Assessment of cellularity, genomic DNA yields, and technical platforms for BRAF mutational testing in thyroid fine-needle aspirate samples / Cancer Cytopa
Forbes SA / 2008 / The Catalogue of Somatic Mutations in Cancer (COSMIC) / Curr Protoc Hum Genet
Hertzman Johansson C / 2014 / BRAF inhibitors in cancer therapy / Pharmacol Ther 142 : 176 ~ 182
Jemal A / 2011 / Global cancer statistics / CA Cancer J Clin 61 : 69 ~ 90
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