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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 408 - 414 (7page)
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초록· 키워드
오류제보하기
Background: The accurate and rapid identification of the causative viruses is important for the timely diagnosis and management of respiratory infections. Multiplex molecular diagnostic techniques have been widely adopted to detect respiratory viruses. We compared the results of a newly upgraded, multiplex, molecular bead-based respiratory viral panel (RVP) assay with the results of Anyplex II RV16 detection kit and AdvanSure RV real-time RT-PCR assay.
Methods: We tested 254 respiratory specimens and cultured viral strains using the Luminex xTAG RVP Fast v2 assay (Luminex Molecular Diagnostics, Canada) and Anyplex II RV16 detection kit and compared the results. Specimens showing discordant results between the two assays were tested with a AdvanSure RV real-time RT-PCR assay.
Results: Of the 254 respiratory specimens, there was total agreement in the results between the xTAG RVP Fast v2 assay and the other real-time PCR assay in 94.1–100% of the specimens. The agreement levels were relatively low (94.1–97.6%) for specimens of adenovirus, coronavirus NL63, and parainfluenza type 3. In comparison to the other assay, the xTAG RVP Fast v2 assay detected a higher number of parainfluenza type 3 (4 cases) and metapneumovirus (9 cases).
Conclusions: The xTAG RVP Fast v2 assay showed comparable capabilities compared with the other assays; it will be useful for identifying respiratory viral infections in patients with respiratory symptoms. Clinicians should be aware of the characteristics of the assays they use, since different assays show different detectability for each virus.
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등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Charles PG / 2008 / Early diagnosis of lower respiratory tract infections (point-of-care tests) / Curr Opin Pulm Med 14 : 176 ~ 182
Chartrand C / 2012 / Accuracy of rapid influenza diagnostic tests: a meta-analysis / Ann Intern Med 156 : 500 ~ 511
Dabisch-Ruthe M / 2012 / Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, R
Duong VT / 2016 / An evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel assay for the detection of multiple diarrheal pathogens in fecal samples in Vietnam / J C
Garbino J / 2004 / Lower respiratory viral illnesses: improved diagnosis by molecular methods and clinical impact / Am J Respir Crit Care Med 170 : 1197 ~ 1203
Chartrand C / 2012 / Accuracy of rapid influenza diagnostic tests: a meta-analysis / Ann Intern Med 156 : 500 ~ 511
Dabisch-Ruthe M / 2012 / Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, R
Duong VT / 2016 / An evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel assay for the detection of multiple diarrheal pathogens in fecal samples in Vietnam / J C
Garbino J / 2004 / Lower respiratory viral illnesses: improved diagnosis by molecular methods and clinical impact / Am J Respir Crit Care Med 170 : 1197 ~ 1203
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