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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 163 - 168 (6page)
- DOI
- 10.7853/kjvs.2021.44.3.163
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초록· 키워드
오류제보하기
The rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in isolating infected patients and preventing further viral transmission. In this study, we evaluated the clinical diagnostic performances of a commercial real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay (Isopollo® COVID-2 assay, M-monitor, Daegu, Korea) using eighty COVID-19 suspected clinical samples and compared these with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (AllplexTM 2019-nCoV rRT-QPCR Assay, SeeGene, Seoul, Korea). The results of the RRT-LAMP assay targeting the N or RdRp gene of SARS-CoV-2 showed perfect agreement with the RT-qPCR assay results in terms of detection. Furthermore, the RRT-LAMP assay was completed in just within a 20-min reaction time, which is significantly faster than about the 2 h currently required for the RT-qPCR assay, thus enabling prompt decision making regarding the isolation of infected patients. The RRT-LAMP assay will be a valuable tool for rapid, sensitive, and specific detection of SARS-CoV-2 in human or unexpected animal clinical cases.
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참고문헌 (11)
참고문헌 신청
Baek YH / 2020 / Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2 / Emerg Microb Infec 9(1):998~1007
Carter LJ / 2020 / Assay techniques and test development for COVID-19 diagnosis / ACS Cent Sci 6:591~605
Corman VM / 2020 / Detection of 2019 novel coronavirus(2019-nCoV)by real-time RT-PCR / Euro Surveill 25(3):2000045
Kitagawa Y / 2020 / Evaluation of rapid diagnosis of novel coronavirus disease(COVID-19)using loop-mediated isothermal amplification / J Clin Virol 129:104446
Lau YL / 2020 / Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 / Peer J 8:e9278
Carter LJ / 2020 / Assay techniques and test development for COVID-19 diagnosis / ACS Cent Sci 6:591~605
Corman VM / 2020 / Detection of 2019 novel coronavirus(2019-nCoV)by real-time RT-PCR / Euro Surveill 25(3):2000045
Kitagawa Y / 2020 / Evaluation of rapid diagnosis of novel coronavirus disease(COVID-19)using loop-mediated isothermal amplification / J Clin Virol 129:104446
Lau YL / 2020 / Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 / Peer J 8:e9278
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