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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 49 - 55 (7page)
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A bacterial strain with phytase and glucose-1-phosphatase activity was isolated from seawater. The colony was identified as an Enterobacter cloacae strain and named E. cloacae B11. A gene, agpEnB11, coding for an intracellular acid glucose phosphatase was cloned from the strain and sequenced. It comprised 1,242 nucleotides and encoded a polypeptide of 413 amino acids. Recombinant glucose-1-phosphatase (AgpEn) was overexpressed in Escherichia coli and purified using Ni-NTA column under native conditions. Purified protein displayed a single band of 47 kDa on SDS-PAGE. AgpEn hydrolyzed a wide variety of phosphorylated compounds, with high activity for glucose-1-phosphate and glucose-6-phosphate. Optimum pH and temperature for enzyme activity were pH 5.0 and 50°C, respectively. Enzyme activity was stimulated by Ca2+ and Co2+, and inhibited by Cu2+.
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참고문헌 (19)
참고문헌 신청
Bolton PG / 1972 / Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in con-tinuous culture / Biochem J 127 : 87 ~ 96
Cottrill MA / 2002 / Inositol phosphatase activity of the Escherichia coli agp-encoded acid glucose-1-phosphatase / Can J Microbiol 48 : 801 ~ 809
Duff SMG / 1991 / Purification and subcellular localization of an acid phosphatase from Brassica nigra suspension cells. Comparison with phosphoenolpyruvate phosphatase / Pro
Duff SMG / 1994 / The role of acid phosphatase in plant phosphorus metabolism / Physiol Plant 90 : 791 ~ 800
Hass H / 1991 / Penicillium chrysobenum extracellular acid pho-sphatase: purification and biochemical characteriza-tion / Bioche Biophys Acta 1074 : 392 ~ 397
Cottrill MA / 2002 / Inositol phosphatase activity of the Escherichia coli agp-encoded acid glucose-1-phosphatase / Can J Microbiol 48 : 801 ~ 809
Duff SMG / 1991 / Purification and subcellular localization of an acid phosphatase from Brassica nigra suspension cells. Comparison with phosphoenolpyruvate phosphatase / Pro
Duff SMG / 1994 / The role of acid phosphatase in plant phosphorus metabolism / Physiol Plant 90 : 791 ~ 800
Hass H / 1991 / Penicillium chrysobenum extracellular acid pho-sphatase: purification and biochemical characteriza-tion / Bioche Biophys Acta 1074 : 392 ~ 397
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