2-Aminoethoxydiphenyl Borate(2-APB)를 이용한 IP3 수용체억제가 와우유모세포의 Gentamicin에 의한 이독성에 미치는 영향

문정환; 정재윤; 안진철; 성연선; 임은석; 이정구

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자료유형: 학술저널

저자정보: 문정환 정재윤 안진철 성연선 임은석 이정구

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제50권 제5호

발행연도: 2007.1

수록면: 385 - 390 (6page)

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Background and Objectives：Elevated intracellular calcium level is known to play important roles in the apoptotic pathway. IP3 receptor (ligand-gated channels that release Ca2+ from intracellular stores) is emerging as a key site for regulation of apo-ptosis. 2-Aminoethoxydiphenyl borate (2-APB)APB on gentamicin ototoxicity in vitro, using the HEI-OC1 cell line. Materials and Method：HEI-OC1 cells were treated with 100μM gentamicin. Using a CaspACE assay, we measured the caspases-3 activity in the gentamicin treated hair cells with and without 2-APB pre-incubation. We also observed intra-cellular calcium concentrations in HEI-OC1 cels using a confocal micro-scopy (calcium gren-1 stain). Live cel imaging was performed by using fluorescence video-time lapse system. Results：Cytosolic calcium elevation by gentamicin was remarkably inhibited by 2-APB. Caspases-3 activities of gentamicin treated cells APB, caspases-3 activities and cel death of gentamicin treated cels were shown to decrease. Conclusion：2-APB reduces Caspases-3 activity in the gentamicin treated HEI-OC1 cells by inhibi-tion of cytosolic calcium increase. (Korean J Otolaryngol 2007 ;50 :385-90)

#2-APB·Caspase-3·Gentamicin·칼슘·IP3 수용체. #2-APB· Caspase-3· Gentamicin· Calcium· IP3 receptor.

참고문헌 (18)

참고문헌 신청

/ / Genetic evidence for involvement of type 1 5-trisphosphate receptors in signal transduction through the Bcell antigen receptor : 3078 ~ 88

/ 1997 / 2APB, 2-Aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins (1,4,5) P3-induced Ca2_ release 122 (3) : 498 ~ 505

/ 1999 / Dynamic studies of ototoxicity in mature avian auditory epithelium 884 : 389 ~ 409

/ 2000 / Tonotopic variations of calciumsignalling in turtle auditory hair cells 524 : 423 ~ 436

/ 2001 / 2 -minoetho- xydi-phenyl borate affects the inositol 1,4,5-trisphosphate receptor, the in-tracellular Ca2+ pump and the non-specific Ca2+ leak from the non-mitochon

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