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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 41 - 49 (9page)
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초록· 키워드
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In the present study, fast and robust methods for the next generation sequencing (NGS) were developed for analysis of PRRSV full genome sequences, which is a positive sensed RNA virus with a high degree of genetic variability among isolates. Two strains of PRRSVs (VR2332 and VR2332-R) which have been maintained in our laboratory were used to validate our methods and to compare with the sequence registered in GenBank (GenBank accession no. EF536003). The results suggested that both of strains had 100% coverage with the reference; the VR2332 had the coverage depth from minimum 3 to max-imum 23,012, for the VR2332-R from minimum 3 to maximum 41,348, and 22,712 as an average depth. Genomic data produced from the massive sequencing capacities of the NGS have enabled the study of PRRSV at an unprecedented rate and details. Unlike conventional sequence methods which re-quire the knowledge of conserved regions, the NGS allows de novo assembly of the full viral genomes. Therefore, our results suggested that these methods using the NGS massively facilitate the generation of more full genome PRRSV sequences locally as well as nationally in regard of saving time and cost.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Albina E / 1997 / Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview / Vet Microbiol 55:309~316
Ansari IH / 2006 / Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies / J Virol 80:3994~4004
Baron T / 1992 / Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation / Ann Rech Vet 23:161~166
Cavanagh D / 1997 / Nidovirales: a new order comprising Coronaviridae and Arteriviridae / Arch Virol 142:629~633
Chan MC / 2015 / Complete Genome Sequence of a Novel Recombinant GII.Pe_GII.17 Norovirus Strain from Hong Kong in 2015 / Genome Announc 3:e01338~e01315
Ansari IH / 2006 / Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies / J Virol 80:3994~4004
Baron T / 1992 / Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation / Ann Rech Vet 23:161~166
Cavanagh D / 1997 / Nidovirales: a new order comprising Coronaviridae and Arteriviridae / Arch Virol 142:629~633
Chan MC / 2015 / Complete Genome Sequence of a Novel Recombinant GII.Pe_GII.17 Norovirus Strain from Hong Kong in 2015 / Genome Announc 3:e01338~e01315
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