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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 761 - 768 (8page)
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초록· 키워드
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Purpose: Our previous studies have shown that oncostatin M (OSM) promotes trophoblast invasion activity through increased enzyme activity of matrix metalloproteinase (MMP)-2 and -9. We further investigated OSM-induced intracellular signaling mechanismsassociated with these events in the immortalized human trophoblast cell line HTR8/SVneo.
Materials and Methods: We investigated the effects of OSM on RNA and protein expression of MMP-2 and -9 in the first-trimester extravillous trophoblast cell line (HTR8/SVneo) via Western blot. The selective signal transducer and activator of transcription (STAT)3 inhibitor, stattic, STAT3 siRNA, and extracellular signal-regulated kinase (ERK) siRNA were used to investigate STAT3 and ERK activation by OSM. The effects of STAT3 and ERK inhibitors on OSM-induced enzymatic activities of MMP-2 and -9 and invasionactivity were further determined via Western blot and gelatin zymography.
Results: OSM-induced MMP-2 and -9 protein expression was significantly suppressed by STAT3 inhibition with stattic and STAT3 siRNA silencing, whereas the ERK1/2 inhibitor (U0126) and ERK silencing significantly suppressed OSM-induced MMP-2 protein expression. OSM-induced MMP-2 and MMP-9 enzymatic activities were significantly decreased by stattic pretreatment. The increasedinvasion activity induced by OSM was significantly suppressed by STAT3 and ERK1/2 inhibition, though to a greater extentby STAT3 inhibition.
Conclusion: Both STAT3 and ERK signaling pathways are involved in OSM-induced invasion activity of HTR8/SVneo cells. Activationof STAT3 appears to be critical for the OSM-mediated increase in invasiveness of HTR8/SVneo cells.
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참고문헌 (25)
참고문헌 신청
Cohen M / 2006 / Metalloproteinases and human placental invasiveness / Placenta 27:783~793
Ogata I / 2000 / Oncostatin M is produced during pregnancy by decidual cells and stimulates the release of HCG / Mol Hum Reprod 6:750~757
Fitzgerald JS / 2005 / Leukemia inhibitory factor triggers activation of signal transducer and activator of transcription 3, proliferation, invasiveness, and altered protease expression in choriocarcinoma cells / Int J Biochem Cell Biol 37:2284~2296
Ko HS / 2012 / The effects of oncostatin M on trophoblast cells: influence on matrix metalloproteinases-2 and -9, and invasion activity / Placenta 33:908~913
Graham CH / 1993 / Establishment and characterization of first trimester human trophoblast cells with extended lifespan / Exp Cell Res 206:204~211
Ogata I / 2000 / Oncostatin M is produced during pregnancy by decidual cells and stimulates the release of HCG / Mol Hum Reprod 6:750~757
Fitzgerald JS / 2005 / Leukemia inhibitory factor triggers activation of signal transducer and activator of transcription 3, proliferation, invasiveness, and altered protease expression in choriocarcinoma cells / Int J Biochem Cell Biol 37:2284~2296
Ko HS / 2012 / The effects of oncostatin M on trophoblast cells: influence on matrix metalloproteinases-2 and -9, and invasion activity / Placenta 33:908~913
Graham CH / 1993 / Establishment and characterization of first trimester human trophoblast cells with extended lifespan / Exp Cell Res 206:204~211
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