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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 1,619 - 1,626 (8page)
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초록· 키워드
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Purpose: There are currently no consistently effective treatments for the excessive collagen produced by keloid fibroblasts. Previously,we reported that heat shock protein 70 (Hsp70) is up-regulated in keloid fibroblasts and keloid tissue. We, therefore, investigatedwhether Hsp70 is related to excessive collagen production in keloid fibroblasts.
Materials and Methods: We inhibited Hsp70 in keloid fibroblasts by RNA interference and examined the resulting collagen expression.
Thus, we selected small interfering RNAs (siRNAs) specific for human Hsp70, transfected them into keloid fibroblasts, and evaluated the resulting phenotypes and protein production using real-time polymerase chain reaction (PCR), Western blot, and a collagen assay.
Results: The siRNAs dramatically suppressed Hsp70 mRNA expression, resulting in a decrease in collagen production in the keloidfibroblasts compared with controls. The siRNAs did not influence the viability of the keloid fibroblasts.
Conclusion: Hsp70 overexpression likely plays an important role in the excessive collagen production by keloid fibroblasts. RNA interference has therapeutic potential for the treatment of keloids.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Ala-Kokko L / 1987 / Collagen gene expression in keloids: analysis of collagen metabolism and type I, III, IV, and V procollagen mRNAs in keloid tissue and keloid fibroblast
Asano T / 2009 / HSP70 confers protection against indomethacin-induced lesions of the small intestine / J Pharmacol Exp Ther 330 : 458 ~ 467
Chen JJ / 2007 / Effect of heat shock protein 47 on collagen accumulation in keloid fibroblast cells / Br J Dermatol 156 : 1188 ~ 1195
Chen W / 2003 / Analysis of differentially expressed genes in keloids and normal skin with cDNA microarray / J Surg Res 113 : 208 ~ 216
Chen W / 2004 / Development of gene microarray in screening differently expressed genes in keloid and normal-control skin / Chin Med J (Engl) 117 : 877 ~ 881
Asano T / 2009 / HSP70 confers protection against indomethacin-induced lesions of the small intestine / J Pharmacol Exp Ther 330 : 458 ~ 467
Chen JJ / 2007 / Effect of heat shock protein 47 on collagen accumulation in keloid fibroblast cells / Br J Dermatol 156 : 1188 ~ 1195
Chen W / 2003 / Analysis of differentially expressed genes in keloids and normal skin with cDNA microarray / J Surg Res 113 : 208 ~ 216
Chen W / 2004 / Development of gene microarray in screening differently expressed genes in keloid and normal-control skin / Chin Med J (Engl) 117 : 877 ~ 881
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