지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1 - 10 (10page)
이용수
초록· 키워드
오류제보하기
Long-term spaceflight affects numerous organ systems in the body, including metabolic dysfunction. Recently, ample evidence has demonstrated that the liver is a vulnerable organ during spaceflight. However, the changes in hepatocyte proliferation and cell cycle control under microgravity remain largely unexplored. In the present study, we first confirmed that the serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase, biochemical markers of liver function, were altered in rats under tail suspension (TS) conditions to simulate microgravity, as shown in previous reports. Next, we demonstrated that the cell proliferation activity, determined by Ki67, PCNA and PH3, was significantly decreased at the different TS time points (TS for 14, 28 and 42 days) compared with that in the control group. Consistently, the positive cell cycle regulators Ccna2, Ccnd1, Cdk1, Cdk2 and cyclin D3 were also significantly decreased in the TS groups as shown by quantitative real-time PCR and western blotting analysis. Subsequent analysis revealed that the aberrant hepatocyte proliferation inhibition under simulated microgravity was associated with the upregulation of miR-223 in the liver. We further found that miR-223 inhibited the proliferation of Hepa1-6 cells and identified CDK2 and CUL1 as its direct targets. In addition, the decreased expression of CDK2 and CUL1 was negatively correlated with the level of p27 in vitro and in vivo, which may have been responsible for retarding hepatocyte proliferation. Collectively, these data indicate that upregulation of miR-223 was associated with the inhibition of liver cell growth and reveal the role of miR-223 in rat hepatocyte proliferation disorders and the pathophysiological process under simulated microgravity.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Bei Y / 2016 / miR-382 targeting PTEN-Akt axis promotes liver regeneration / Oncotarget 7 : 1584 ~ 1597
Chen X / 2016 / MicroRNA-21 contributes to liver regeneration by targeting PTEN / Med Sci Monit 22 : 83 ~ 91
Cressman DE / 1996 / Liver failure and defective hepatocyte regeneration in interleukin-6-deficient mice / Science 274 : 1379 ~ 1383
Du F / 2015 / Morphology and molecular mechanisms of hepatic injury in rats under simulated weightlessness and the protective effects of resistance training / PloS ONE 10 : e
Fausto N / 2006 / Liver regeneration / Hepatology 43 : S45 ~ S53
Chen X / 2016 / MicroRNA-21 contributes to liver regeneration by targeting PTEN / Med Sci Monit 22 : 83 ~ 91
Cressman DE / 1996 / Liver failure and defective hepatocyte regeneration in interleukin-6-deficient mice / Science 274 : 1379 ~ 1383
Du F / 2015 / Morphology and molecular mechanisms of hepatic injury in rats under simulated weightlessness and the protective effects of resistance training / PloS ONE 10 : e
Fausto N / 2006 / Liver regeneration / Hepatology 43 : S45 ~ S53
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Hepatocyte organoids and cell transplantation: What the future holds
Experimental and Molecular Medicine
2021 .10
-
MiR-371 promotes proliferation and metastasis in hepatocellular carcinoma by targeting PTEN
BMB Reports
2019 .01
-
Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury
Experimental and Molecular Medicine
2022 .11
-
MiR-34a and miR-34b/c have distinct effects on the suppression of lung adenocarcinomas
Experimental and Molecular Medicine
2019 .01
-
Gene expression regulation by CDK12: a versatile kinase in cancer with functions beyond CTD phosphorylation
Experimental and Molecular Medicine
2020 .01
-
miR-30c regulates proliferation, apoptosis and differentiation via the Shh signaling pathway in P19 cells
Experimental and Molecular Medicine
2016 .01
-
A Novel Approach to Modeled Microgravity Cell Culture Using Neutral Buoyancy
한국고분자학회 학술대회 연구논문 초록집
2024 .09
-
MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort
Experimental and Molecular Medicine
2015 .01
-
miR-31a-5p promotes postnatal cardiomyocyte proliferation by targeting RhoBTB1
Experimental and Molecular Medicine
2017 .01
-
ANT2 shRNA downregulates miR-19a and miR-96 through the PI3K/Akt pathway and suppresses tumor growth in hepatocellular carcinoma cells
Experimental and Molecular Medicine
2016 .01
-
MicroRNA-103a-3p controls proliferation and osteogenic differentiation of human adipose tissue-derived stromal cells
Experimental and Molecular Medicine
2015 .01
-
Role of miR-223 in the pathophysiology of liver diseases
Experimental and Molecular Medicine
2018 .01
-
NMR hydrogen exchange study of miR156:miR156* duplexes
Journal of the Korean Magnetic Resonance Society
2019 .01
-
Transcriptional Profiling of the Probiotic Escherichia coli Nissle 1917 Strain under Simulated Microgravity
한국생물공학회 학술대회
2023 .10
-
MicroRNA-375 is a therapeutic target for castration-resistant prostate cancer through the PTPN4/STAT3 axis
Experimental and Molecular Medicine
2022 .08
-
Effect of Microgravity on Stem Cell Proliferation
대한기계학회 춘추학술대회
2019 .04
-
MiR-125a-5p ameliorates monocrotalineinduced pulmonary arterial hypertension by targeting the TGF-β1 and IL-6/STAT3 signaling pathways
Experimental and Molecular Medicine
2018 .01
-
miR-638 is a new biomarker for outcome prediction of non-small cell lung cancer patients receiving chemotherapy
Experimental and Molecular Medicine
2015 .01
-
Microgravity Separation of Microparticles through Microfluidic System
한국생물공학회 학술대회
2015 .04
-
MicroRNA-301b promotes cell proliferation and apoptosis resistance in triple-negative breast cancer by targeting CYLD
BMB Reports
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0