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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 824 - 831 (8page)
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초록· 키워드
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Hu protein R (HuR) binds to the AU-rich element (ARE)
in the 3'UTR to stabilize TNF-α mRNA. Here, we identified
chemical inhibitors of the interaction between HuR
and the ARE of TNF-α mRNA using RNA electrophoretic
mobility gel shift assay (EMSA) and filter binding
assay. Of 179 chemicals screened, we identified
three with a half-maximal inhibitory concentration
(IC50) below 10 μM. The IC50 of quercetin, b-40, and b-41
were 1.4, 0.38, and 6.21 μM, respectively, for binding of
HuR protein to TNF-α mRNA. Quercetin and b-40 did
not inhibit binding of tristetraprolin to the ARE of TNF-α
mRNA. When LPS-treated RAW264.7 cells were treated
with quercetin and b-40, we observed decreased stability
of TNF-α mRNA and decreased levels of secreted
TNF-α. From these results, we could find inhibitors for
the TNF-α mRNA stability, which might be used advantageously
for both the study for post-transcriptional
regulation and the discovery of new anti-inflammation
drugs.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Beutler B / 1989 / The biology of cachectin/TNF--a primary mediator of the host response / Annu Rev Immunol 7 : 625 ~ 655
Brennan CM / 2001 / HuR and mRNA stability / Cell Mol Life Sci 58 : 266 ~ 277
Dean JL / 2001 / The 3' untranslated region of tumor necrosis factor alpha mRNA is a target of the mRNA-stabilizing factor HuR / Mol Cell Biol 21 : 721 ~ 730
Fan J / 2005 / The role of post-transcriptional regulation in chemokine gene expression in inflammation and allergy / Eur Respir J 26 : 933 ~ 947
Fan XC / 1998 / Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs / EMBO J 17 : 3448 ~ 3460
Brennan CM / 2001 / HuR and mRNA stability / Cell Mol Life Sci 58 : 266 ~ 277
Dean JL / 2001 / The 3' untranslated region of tumor necrosis factor alpha mRNA is a target of the mRNA-stabilizing factor HuR / Mol Cell Biol 21 : 721 ~ 730
Fan J / 2005 / The role of post-transcriptional regulation in chemokine gene expression in inflammation and allergy / Eur Respir J 26 : 933 ~ 947
Fan XC / 1998 / Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs / EMBO J 17 : 3448 ~ 3460
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